TY - JOUR

T1 - Comparison of characteristics of human small cell carcinoma of the lung in patients, in vitro and transplanted into nude mice

AU - Engelholm, S A

AU - Spang-Thomsen, M

AU - Vindeløv, L L

AU - Brünner, N

AU - Nielsen, M H

AU - Hirsch, F

AU - Nielsen, A

AU - Hansen, H H

N1 - Keywords: Animals; Carcinoma, Small Cell; Cell Count; Cells, Cultured; DNA, Neoplasm; Humans; Lung Neoplasms; Mice; Mice, Nude; Neoplasm Transplantation

PY - 1986

Y1 - 1986

N2 - Specimens from 24 patients with metastatic small cell carcinoma of the lung were explanted in vitro as well as transplanted directly into nude mice. A method to obtain fibroblast-free cultures is described. This method resulted in cell lines which could be grown for more than one year in 79% of the cases. Fifty-four % of the tumours could be established as serially transplantable tumours in nude mice. The tumours were characterized by histology, electron microscopy, DNA index, and cell cycle distribution. The in vitro cell lines were furthermore characterized by the plating efficiency and by doubling time. The macroscopic growth of the heterotransplanted tumours was ascribed to a transformed Gompertz function. The tumour cells preserved their light microscopic constitution of small cell carcinoma of the lung in the model systems. The heterogeneity of the original tumours was reflected in vitro and in nude mice and the model systems thus allows an expression of the inherent heterogeneity and instability. The panel of transplantable tumours and the in vitro cell lines offer the study of biology inclusive of tumour progression of SCCL.

AB - Specimens from 24 patients with metastatic small cell carcinoma of the lung were explanted in vitro as well as transplanted directly into nude mice. A method to obtain fibroblast-free cultures is described. This method resulted in cell lines which could be grown for more than one year in 79% of the cases. Fifty-four % of the tumours could be established as serially transplantable tumours in nude mice. The tumours were characterized by histology, electron microscopy, DNA index, and cell cycle distribution. The in vitro cell lines were furthermore characterized by the plating efficiency and by doubling time. The macroscopic growth of the heterotransplanted tumours was ascribed to a transformed Gompertz function. The tumour cells preserved their light microscopic constitution of small cell carcinoma of the lung in the model systems. The heterogeneity of the original tumours was reflected in vitro and in nude mice and the model systems thus allows an expression of the inherent heterogeneity and instability. The panel of transplantable tumours and the in vitro cell lines offer the study of biology inclusive of tumour progression of SCCL.

M3 - Journal article

C2 - 3020867

VL - 94

SP - 325

EP - 336

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section A Pathology

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section A Pathology

SN - 0108-0164

IS - 5

ER -