Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene

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Standard

Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. / Efrat, S; Linde, S; Kofod, Hans; Spector, D; Delannoy, M; Grant, S; Hanahan, D; Baekkeskov, S.

I: Proceedings of the National Academy of Sciences of the United States of America, Bind 85, Nr. 23, 12.1988, s. 9037-41.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Efrat, S, Linde, S, Kofod, H, Spector, D, Delannoy, M, Grant, S, Hanahan, D & Baekkeskov, S 1988, 'Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene', Proceedings of the National Academy of Sciences of the United States of America, bind 85, nr. 23, s. 9037-41.

APA

Efrat, S., Linde, S., Kofod, H., Spector, D., Delannoy, M., Grant, S., Hanahan, D., & Baekkeskov, S. (1988). Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. Proceedings of the National Academy of Sciences of the United States of America, 85(23), 9037-41.

Vancouver

Efrat S, Linde S, Kofod H, Spector D, Delannoy M, Grant S o.a. Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. Proceedings of the National Academy of Sciences of the United States of America. 1988 dec.;85(23):9037-41.

Author

Efrat, S ; Linde, S ; Kofod, Hans ; Spector, D ; Delannoy, M ; Grant, S ; Hanahan, D ; Baekkeskov, S. / Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene. I: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Bind 85, Nr. 23. s. 9037-41.

Bibtex

@article{53eebc29960e4cfea76f9a1050b1c235,
title = "Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene",
abstract = "Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.",
keywords = "Adenoma, Islet Cell, Animals, Antigens, Viral, Tumor, Genes, Insulin, Insulinoma, Islets of Langerhans, Mice, Mice, Transgenic, Oncogenes, Pancreatic Neoplasms, Simian virus 40, Tumor Cells, Cultured",
author = "S Efrat and S Linde and Hans Kofod and D Spector and M Delannoy and S Grant and D Hanahan and S Baekkeskov",
year = "1988",
month = dec,
language = "English",
volume = "85",
pages = "9037--41",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "23",

}

RIS

TY - JOUR

T1 - Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene

AU - Efrat, S

AU - Linde, S

AU - Kofod, Hans

AU - Spector, D

AU - Delannoy, M

AU - Grant, S

AU - Hanahan, D

AU - Baekkeskov, S

PY - 1988/12

Y1 - 1988/12

N2 - Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.

AB - Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta TC) lines maintain the features of differentiated beta cells for about 50 passages in culture. The cells produce both proinsulin I and II and efficiently process each into mature insulin, in a manner comparable to normal beta cells in isolated islets. Electron microscopy reveals typical beta-cell type secretory granules, in which insulin is stored. Insulin secretion is inducible up to 30-fold by glucose, although with a lower threshold for maximal stimulation than that for normal beta cells. beta TC lines can be repeatedly derived from primary beta-cell tumors that heritably arise in the transgenic mice. Thus, targeted expression of an oncogene with a cell-specific regulatory element can be used both to immortalize a rare cell type and to provide a selection for the maintenance of its differentiated phenotype.

KW - Adenoma, Islet Cell

KW - Animals

KW - Antigens, Viral, Tumor

KW - Genes

KW - Insulin

KW - Insulinoma

KW - Islets of Langerhans

KW - Mice

KW - Mice, Transgenic

KW - Oncogenes

KW - Pancreatic Neoplasms

KW - Simian virus 40

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 2848253

VL - 85

SP - 9037

EP - 9041

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -

ID: 45575125