A reporter of UV intensity delivered to the cytosol during photolytic uncaging

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Standard

A reporter of UV intensity delivered to the cytosol during photolytic uncaging. / Brasen, Jens Christian; Dewitt, Sharon; Hallett, Maurice B.

I: Biophysical Journal, Bind 98, Nr. 7, 07.04.2010, s. L25-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Brasen, JC, Dewitt, S & Hallett, MB 2010, 'A reporter of UV intensity delivered to the cytosol during photolytic uncaging', Biophysical Journal, bind 98, nr. 7, s. L25-7. https://doi.org/10.1016/j.bpj.2009.12.4271

APA

Brasen, J. C., Dewitt, S., & Hallett, M. B. (2010). A reporter of UV intensity delivered to the cytosol during photolytic uncaging. Biophysical Journal, 98(7), L25-7. https://doi.org/10.1016/j.bpj.2009.12.4271

Vancouver

Brasen JC, Dewitt S, Hallett MB. A reporter of UV intensity delivered to the cytosol during photolytic uncaging. Biophysical Journal. 2010 apr. 7;98(7):L25-7. https://doi.org/10.1016/j.bpj.2009.12.4271

Author

Brasen, Jens Christian ; Dewitt, Sharon ; Hallett, Maurice B. / A reporter of UV intensity delivered to the cytosol during photolytic uncaging. I: Biophysical Journal. 2010 ; Bind 98, Nr. 7. s. L25-7.

Bibtex

@article{ecfd5871b6b44931a3195e800deda815,
title = "A reporter of UV intensity delivered to the cytosol during photolytic uncaging",
abstract = "Photolytic uncaging of biologically-active molecules within cells is a powerful technique. However, the delivery of uncaging light into the cytosol can vary between cell types, individual cells of the same type, and different loci within an individual cell because of optical differences in absorbance and light-scattering properties of the cytoplasm. Here, we demonstrate a simple technique for monitoring the magnitude of cytosolic ultraviolet delivery during uncaging, which also leaves a quantitative and persistent record of this within the cells. The simple method shown here provides a much needed universal monitor of the delivery of ultraviolet light to molecules within the cytosol, providing a much needed parameter for the correct interpretation of uncaging experiments.",
keywords = "Animals, Biophysics, Cell Line, Cytoplasm, Cytosol, HL-60 Cells, Humans, Light, Mice, Models, Chemical, NAD, Oxygen, Photolysis, Scattering, Radiation, Ultraviolet Rays",
author = "Brasen, {Jens Christian} and Sharon Dewitt and Hallett, {Maurice B}",
note = "Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.",
year = "2010",
month = apr,
day = "7",
doi = "10.1016/j.bpj.2009.12.4271",
language = "English",
volume = "98",
pages = "L25--7",
journal = "Biophysical Journal",
issn = "0006-3495",
publisher = "Cell Press",
number = "7",

}

RIS

TY - JOUR

T1 - A reporter of UV intensity delivered to the cytosol during photolytic uncaging

AU - Brasen, Jens Christian

AU - Dewitt, Sharon

AU - Hallett, Maurice B

N1 - Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.

PY - 2010/4/7

Y1 - 2010/4/7

N2 - Photolytic uncaging of biologically-active molecules within cells is a powerful technique. However, the delivery of uncaging light into the cytosol can vary between cell types, individual cells of the same type, and different loci within an individual cell because of optical differences in absorbance and light-scattering properties of the cytoplasm. Here, we demonstrate a simple technique for monitoring the magnitude of cytosolic ultraviolet delivery during uncaging, which also leaves a quantitative and persistent record of this within the cells. The simple method shown here provides a much needed universal monitor of the delivery of ultraviolet light to molecules within the cytosol, providing a much needed parameter for the correct interpretation of uncaging experiments.

AB - Photolytic uncaging of biologically-active molecules within cells is a powerful technique. However, the delivery of uncaging light into the cytosol can vary between cell types, individual cells of the same type, and different loci within an individual cell because of optical differences in absorbance and light-scattering properties of the cytoplasm. Here, we demonstrate a simple technique for monitoring the magnitude of cytosolic ultraviolet delivery during uncaging, which also leaves a quantitative and persistent record of this within the cells. The simple method shown here provides a much needed universal monitor of the delivery of ultraviolet light to molecules within the cytosol, providing a much needed parameter for the correct interpretation of uncaging experiments.

KW - Animals

KW - Biophysics

KW - Cell Line

KW - Cytoplasm

KW - Cytosol

KW - HL-60 Cells

KW - Humans

KW - Light

KW - Mice

KW - Models, Chemical

KW - NAD

KW - Oxygen

KW - Photolysis

KW - Scattering, Radiation

KW - Ultraviolet Rays

U2 - 10.1016/j.bpj.2009.12.4271

DO - 10.1016/j.bpj.2009.12.4271

M3 - Journal article

C2 - 20371308

VL - 98

SP - L25-7

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

IS - 7

ER -

ID: 33812918