A Kir2.1 gain-of-function mutation underlies familial atrial fibrillation
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A Kir2.1 gain-of-function mutation underlies familial atrial fibrillation. / Xia, Min; Jin, Qingfeng; Bendahhou, Saïd; He, Yusong; Larroque, Marie-Madeleine; Chen, Yiping; Zhou, Qinshu; Yang, Yiqing; Liu, Yi; Liu, Ban; Zhu, Qian; Zhou, Yanting; Lin, Jie; Liang, Bo; Li, Li; Dong, Xiongjian; Pan, Zhiwen; Wang, Rongrong; Wan, Haiying; Qiu, Weiqin; Xu, Wenyuan; Eurlings, Petra; Barhanin, Jacques; Chen, Yihan.
I: Biochemical and Biophysical Research Communications, Bind 332, Nr. 4, 2005, s. 1012-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - A Kir2.1 gain-of-function mutation underlies familial atrial fibrillation
AU - Xia, Min
AU - Jin, Qingfeng
AU - Bendahhou, Saïd
AU - He, Yusong
AU - Larroque, Marie-Madeleine
AU - Chen, Yiping
AU - Zhou, Qinshu
AU - Yang, Yiqing
AU - Liu, Yi
AU - Liu, Ban
AU - Zhu, Qian
AU - Zhou, Yanting
AU - Lin, Jie
AU - Liang, Bo
AU - Li, Li
AU - Dong, Xiongjian
AU - Pan, Zhiwen
AU - Wang, Rongrong
AU - Wan, Haiying
AU - Qiu, Weiqin
AU - Xu, Wenyuan
AU - Eurlings, Petra
AU - Barhanin, Jacques
AU - Chen, Yihan
PY - 2005
Y1 - 2005
N2 - The inward rectifier K(+) channel Kir2.1 mediates the potassium I(K1) current in the heart. It is encoded by KCNJ2 gene that has been linked to Andersen's syndrome. Recently, strong evidences showed that Kir2.1 channels were associated with mouse atrial fibrillation (AF), therefore we hypothesized that KCNJ2 was associated with familial AF. Thirty Chinese AF kindreds were evaluated for mutations in KCNJ2 gene. A valine-to-isoleucine mutation at position 93 (V93I) of Kir2.1 was found in all affected members in one kindred. This valine and its flanking sequence is highly conserved in Kir2.1 proteins among different species. Functional analysis of the V93I mutant demonstrated a gain-of-function consequence on the Kir2.1 current. This effect is opposed to the loss-of-function effect of previously reported mutations in Andersen's syndrome. Kir2.1 V93I mutation may play a role in initiating and/or maintaining AF by increasing the activity of the inward rectifier K(+) channel.
AB - The inward rectifier K(+) channel Kir2.1 mediates the potassium I(K1) current in the heart. It is encoded by KCNJ2 gene that has been linked to Andersen's syndrome. Recently, strong evidences showed that Kir2.1 channels were associated with mouse atrial fibrillation (AF), therefore we hypothesized that KCNJ2 was associated with familial AF. Thirty Chinese AF kindreds were evaluated for mutations in KCNJ2 gene. A valine-to-isoleucine mutation at position 93 (V93I) of Kir2.1 was found in all affected members in one kindred. This valine and its flanking sequence is highly conserved in Kir2.1 proteins among different species. Functional analysis of the V93I mutant demonstrated a gain-of-function consequence on the Kir2.1 current. This effect is opposed to the loss-of-function effect of previously reported mutations in Andersen's syndrome. Kir2.1 V93I mutation may play a role in initiating and/or maintaining AF by increasing the activity of the inward rectifier K(+) channel.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Amino Acid Sequence
KW - Animals
KW - Atrial Fibrillation
KW - COS Cells
KW - Cell Line
KW - Conserved Sequence
KW - DNA
KW - DNA Mutational Analysis
KW - Electrophysiology
KW - Family Health
KW - Female
KW - Heart Atria
KW - Humans
KW - Isoleucine
KW - Male
KW - Microscopy, Confocal
KW - Middle Aged
KW - Models, Molecular
KW - Molecular Sequence Data
KW - Mutagenesis, Site-Directed
KW - Mutation
KW - Potassium Channels, Inwardly Rectifying
KW - Protein Conformation
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Sequence Analysis, DNA
KW - Transfection
KW - Valine
U2 - 10.1016/j.bbrc.2005.05.054
DO - 10.1016/j.bbrc.2005.05.054
M3 - Journal article
C2 - 15922306
VL - 332
SP - 1012
EP - 1019
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -
ID: 38440003