Quantification of protein modification by oxidants
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Quantification of protein modification by oxidants. / Hawkins, Clare Louise; Morgan, Philip E; Davies, Michael Jonathan.
In: Free Radical Biology & Medicine, Vol. 46, No. 8, 15.04.2009, p. 965-88.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Quantification of protein modification by oxidants
AU - Hawkins, Clare Louise
AU - Morgan, Philip E
AU - Davies, Michael Jonathan
PY - 2009/4/15
Y1 - 2009/4/15
N2 - Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.
AB - Proteins are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species, such as singlet oxygen. Exposure of proteins to these oxidants results in loss of the parent amino acid residue, formation of unstable intermediates, and the generation of stable products. Each of these events can be used, to a greater or lesser extent, to quantify damage to proteins. In this review the advantages and disadvantages of a number of these approaches are discussed, with an emphasis on methods that yield absolute quantitative data on the extent of protein modification. Detailed methods sheets are provided for many of these techniques.
KW - Animals
KW - Biochemistry
KW - Biological Markers
KW - Chromatography, High Pressure Liquid
KW - Enzyme-Linked Immunosorbent Assay
KW - Humans
KW - Mass Spectrometry
KW - Oxidants
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Protein Processing, Post-Translational
KW - Proteins
KW - Reactive Oxygen Species
KW - Spin Trapping
KW - Statistics as Topic
U2 - 10.1016/j.freeradbiomed.2009.01.007
DO - 10.1016/j.freeradbiomed.2009.01.007
M3 - Journal article
C2 - 19439229
VL - 46
SP - 965
EP - 988
JO - Free Radical Biology & Medicine
JF - Free Radical Biology & Medicine
SN - 0891-5849
IS - 8
ER -
ID: 129670374