Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

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Standard

Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides. / Wehner, K; Fritz, P; Köhler, K; Multhaupt, H.

I: Acta histochemica. Supplementband, Bind 37, 1989, s. 83-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Wehner, K, Fritz, P, Köhler, K & Multhaupt, H 1989, 'Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.', Acta histochemica. Supplementband, bind 37, s. 83-8.

APA

Wehner, K., Fritz, P., Köhler, K., & Multhaupt, H. (1989). Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides. Acta histochemica. Supplementband, 37, 83-8.

Vancouver

Wehner K, Fritz P, Köhler K, Multhaupt H. Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides. Acta histochemica. Supplementband. 1989;37:83-8.

Author

Wehner, K ; Fritz, P ; Köhler, K ; Multhaupt, H. / Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides. I: Acta histochemica. Supplementband. 1989 ; Bind 37. s. 83-8.

Bibtex

@article{a33515605d4311dd8d9f000ea68e967b,
title = "Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.",
abstract = "HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.",
author = "K Wehner and P Fritz and K K{\"o}hler and H Multhaupt",
note = "Keywords: Hela Cells; Humans; Immunoenzyme Techniques; Neoplasm Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Poly A; RNA, Messenger",
year = "1989",
language = "English",
volume = "37",
pages = "83--8",
journal = "Acta histochemica. Supplementband",
issn = "0567-7556",

}

RIS

TY - JOUR

T1 - Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

AU - Wehner, K

AU - Fritz, P

AU - Köhler, K

AU - Multhaupt, H

N1 - Keywords: Hela Cells; Humans; Immunoenzyme Techniques; Neoplasm Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Poly A; RNA, Messenger

PY - 1989

Y1 - 1989

N2 - HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

AB - HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.

M3 - Journal article

C2 - 2505326

VL - 37

SP - 83

EP - 88

JO - Acta histochemica. Supplementband

JF - Acta histochemica. Supplementband

SN - 0567-7556

ER -

ID: 5240525