Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
Originalsprog | Engelsk |
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Tidsskrift | FEBS Letters |
Vol/bind | 235 |
Udgave nummer | 1-2 |
Sider (fra-til) | 122-124 |
Antal sider | 3 |
ISSN | 0014-5793 |
DOI | |
Status | Udgivet - 1 aug. 1988 |
ID: 203631297