Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts
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Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts. / Nielsen, Peter E.; Jeppesen, Claus; Buchardt, O.
I: FEBS Letters, Bind 235, Nr. 1-2, 01.08.1988, s. 122-124.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Uranyl salts as photochemical agents for cleavage of DNA and probing of protein-DNA contacts
AU - Nielsen, Peter E.
AU - Jeppesen, Claus
AU - Buchardt, O
PY - 1988/8/1
Y1 - 1988/8/1
N2 - Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
AB - Single-strand DNA nicks are induced by uranyl nitrate or uranyl acetate in combination with long-wavelength (lambda approximately 420 nm) ultraviolet irradiation. The nicks occur randomly with respect to the DNA sequence. Using the lambda-repressor/ORI operator DNA system it is shown that uranyl salts can be used to photofootprint protein contacts with the DNA backbone.
KW - Bacteriophage lambda/analysis
KW - Base Sequence
KW - DNA/drug effects
KW - DNA, Superhelical/drug effects
KW - Electrophoresis, Polyacrylamide Gel
KW - Operator Regions, Genetic
KW - Organometallic Compounds/pharmacology
KW - Photochemistry
KW - Plasmids
KW - Proteins/metabolism
KW - Repressor Proteins/metabolism
KW - Ultraviolet Rays
KW - Uranium/pharmacology
KW - Uranyl Nitrate/pharmacology
U2 - 10.1016/0014-5793(88)81245-6
DO - 10.1016/0014-5793(88)81245-6
M3 - Journal article
C2 - 2969827
VL - 235
SP - 122
EP - 124
JO - F E B S Letters
JF - F E B S Letters
SN - 0014-5793
IS - 1-2
ER -
ID: 203631297