Uranyl photoprobing of conformational changes in DNA induced by drug binding
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Uranyl photoprobing of conformational changes in DNA induced by drug binding. / Nielsen, Peter E.; Møllegaard, N E; Jeppesen, C.
I: Anti-Cancer Drug Design, Bind 5, Nr. 1, 02.1990, s. 105-10.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Uranyl photoprobing of conformational changes in DNA induced by drug binding
AU - Nielsen, Peter E.
AU - Møllegaard, N E
AU - Jeppesen, C
PY - 1990/2
Y1 - 1990/2
N2 - The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.
AB - The effect on DNA conformation upon binding of the bis-intercalator, N,N'-diacridinyl spermidine is studied by uranyl mediated DNA photocleavage and gel retardation. It is shown that a characteristic A-tract correlated 10 base pair modulation of the uranyl photocleavage of bent (kinetoplast) DNA is suppressed upon binding of the diacridine. Likewise, the anomalous slow gel electrophoretic migration of bent-DNA is abolished by diacridine binding. At higher diacridine/DNA ratios a DNA conformation in which G-residues become hypersensitive to uranyl photocleavage is induced.
KW - DNA/analysis
KW - DNA Probes/pharmacology
KW - DNA, Circular/analysis
KW - DNA, Kinetoplast
KW - Electrophoresis, Polyacrylamide Gel
KW - In Vitro Techniques
KW - Nucleic Acid Conformation/drug effects
KW - Photochemistry
M3 - Journal article
C2 - 2156513
VL - 5
SP - 105
EP - 110
JO - Anti-Cancer Drug Design
JF - Anti-Cancer Drug Design
SN - 0266-9536
IS - 1
ER -
ID: 203632167