Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia?

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Standard

Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia? / Hansen, A M K; Bödvarsdottir, T B; Nordestgaard, D N E; Heller, R S; Gotfredsen, C F; Maedler, K; Fels, JJ; Holst, Jens Juul; Karlsen, AE.

I: Diabetologia, Bind 54, Nr. 6, 2011, s. 1379-87.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hansen, AMK, Bödvarsdottir, TB, Nordestgaard, DNE, Heller, RS, Gotfredsen, CF, Maedler, K, Fels, JJ, Holst, JJ & Karlsen, AE 2011, 'Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia?', Diabetologia, bind 54, nr. 6, s. 1379-87. https://doi.org/10.1007/s00125-011-2080-1

APA

Hansen, A. M. K., Bödvarsdottir, T. B., Nordestgaard, D. N. E., Heller, R. S., Gotfredsen, C. F., Maedler, K., Fels, JJ., Holst, J. J., & Karlsen, AE. (2011). Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia? Diabetologia, 54(6), 1379-87. https://doi.org/10.1007/s00125-011-2080-1

Vancouver

Hansen AMK, Bödvarsdottir TB, Nordestgaard DNE, Heller RS, Gotfredsen CF, Maedler K o.a. Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia? Diabetologia. 2011;54(6):1379-87. https://doi.org/10.1007/s00125-011-2080-1

Author

Hansen, A M K ; Bödvarsdottir, T B ; Nordestgaard, D N E ; Heller, R S ; Gotfredsen, C F ; Maedler, K ; Fels, JJ ; Holst, Jens Juul ; Karlsen, AE. / Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia?. I: Diabetologia. 2011 ; Bind 54, Nr. 6. s. 1379-87.

Bibtex

@article{18d480406c3643e19eb008731c9d06ee,

title = "Upregulation of alpha cell glucagon-like peptide 1 (GLP-1) in Psammomys obesus--an adaptive response to hyperglycaemia?",

abstract = "Aims/hypothesis The hormone glucagon-like peptide 1 (GLP-1) is released in response to a meal from the intestinal L-cells, where it is processed from proglucagon by the proconvertase (PC)1/3. In contrast, in the adult islets proglucagon is processed to glucagon by the PC2 enzyme. The aim of the study was to evaluate if, during the development of diabetes, alpha cells produce GLP-1 that, in turn, might trigger beta cell growth. Methods Beta cell mass, GLP-1 and insulin levels were measured in the gerbil Psammomys obesus (P. obesus), a rodent model of nutritionally induced diabetes. Furthermore, the presence of biologically active forms of GLP-1 and PC1/3 in alpha cells was demonstrated by immunofluorescence, and the release of GLP-1 from isolated P. obesus, mouse and human islets was investigated. Results During the development of diabetes in P. obesus, a significant increase in GLP-1 was detected in the portal vein (9.8¿±¿1.5 vs 4.3¿±¿0.7 pmol/l, p¿<¿0.05), and in pancreas extracts (11.4¿±¿2.2 vs 5.1¿±¿1.3 pmol/g tissue, p¿<¿0.05). Freshly isolated islets from hyperglycaemic animals released more GLP-1 following 24 h culture than islets from control animals (28.2¿±¿4.4 pmol/l vs 5.8¿±¿2.4, p¿<¿0.01). GLP-1 release was increased from healthy P. obesus islets following culture in high glucose for 6 days (91¿±¿9.1 pmol/l vs 28.8¿±¿6.6, p¿<¿0.01). High levels of GLP-1 were also found to be released from human islets. PC1/3 colocalised weakly with alpha cells. Conclusions/interpretation GLP-1 release from alpha cells is upregulated in P. obesus during the development of diabetes. A similar response is seen in islets exposed to high glucose, which supports the hypothesis that GLP-1 released from alpha cells promotes an increase in beta cell mass and function during metabolic challenge such as diabetes. ",

keywords = "Adaptation, Physiological, Adult, Animals, Cell Proliferation, Cells, Cultured, Diabetes Mellitus, Diet, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Gerbillinae, Glucagon-Like Peptide 1, Glucagon-Secreting Cells, Glucose, Humans, Hyperglycemia, Insulin, Insulin-Secreting Cells, Islets of Langerhans, Male, Mice, Middle Aged, Obesity, Up-Regulation",

author = "Hansen, {A M K} and B{\"o}dvarsdottir, {T B} and Nordestgaard, {D N E} and Heller, {R S} and Gotfredsen, {C F} and K Maedler and JJ Fels and Holst, {Jens Juul} and AE Karlsen",

year = "2011",

doi = "10.1007/s00125-011-2080-1",

language = "English",

volume = "54",

pages = "1379--87",

journal = "Diabetologia",

issn = "0012-186X",

publisher = "Springer",

number = "6",