The prevalence of controls in phyllosphere microbiome research: a methodological review
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DNA contamination can critically confound microbiome studies. Here, we take a systematic approach to review the current literature and investigate the prevalence of contamination controls in phyllosphere microbiome research over the past decade. By utilising systematic review principles for this review, we were able to conduct a thorough investigation, screening 450 articles from three databases for eligibility and extracting data in a controlled and methodical manner. Worryingly, we observed a surprisingly low usage of both positive and negative contamination controls in phyllosphere research. As a result, we propose a set of minimum standards to combat the effects of contamination in future phyllosphere research.
Originalsprog | Engelsk |
---|---|
Tidsskrift | New Phytologist |
Vol/bind | 242 |
Udgave nummer | 1 |
Sider (fra-til) | 23-29 |
Antal sider | 7 |
ISSN | 0028-646X |
DOI | |
Status | Udgivet - 2024 |
Bibliografisk note
Funding Information:
This work was supported by Wine Australia, with levies from Australia's grape growers and winemakers and matching funds from the Australian Government. Open access publishing facilitated by The University of Adelaide, as part of the Wiley ‐ The University of Adelaide agreement via the Council of Australian University Librarians.
Publisher Copyright:
© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.
ID: 383733910