The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes
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The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes. / Nexø, Ebba; Poulsen, Steen Seier.
I: BBA General Subjects, Bind 1074, Nr. 2, 08.07.1991, s. 284-88.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - The membrane fraction of homogenized rat kidney contains an enzyme that releases epidermal growth factor from the kidney membranes
AU - Nexø, Ebba
AU - Poulsen, Steen Seier
PY - 1991/7/8
Y1 - 1991/7/8
N2 - High levels of epidermal growth factor (EGF) are excreted in the urine and high levels of mRNA for the EGF-precursor have been demonstrated in the kidney. The EGF-precursor is a membrane bound peptide in the kidney, but little is known about the renal processing of the precursor. The present study shows that the membrane fraction of homogenized rat kidney contains an enzyme that releases immuno and receptor reactive EGF from the kidney membranes when incubated at 37 degrees C. Gel filtration shows that the EGF reactivity released from the membranes is similar to the EGF reactivity in rat urine. The EGF releasing enzyme is inhibited by the serine proteinase inhibitor aprotinin and by low temperatures (4 degrees C). The pH optimum of the reaction is pH 7.5-8.0.
AB - High levels of epidermal growth factor (EGF) are excreted in the urine and high levels of mRNA for the EGF-precursor have been demonstrated in the kidney. The EGF-precursor is a membrane bound peptide in the kidney, but little is known about the renal processing of the precursor. The present study shows that the membrane fraction of homogenized rat kidney contains an enzyme that releases immuno and receptor reactive EGF from the kidney membranes when incubated at 37 degrees C. Gel filtration shows that the EGF reactivity released from the membranes is similar to the EGF reactivity in rat urine. The EGF releasing enzyme is inhibited by the serine proteinase inhibitor aprotinin and by low temperatures (4 degrees C). The pH optimum of the reaction is pH 7.5-8.0.
KW - Animals
KW - Chromatography, Gel
KW - Enzyme-Linked Immunosorbent Assay
KW - Epidermal Growth Factor
KW - Hydrogen-Ion Concentration
KW - Kidney
KW - Male
KW - Radioligand Assay
KW - Rats
KW - Rats, Inbred Strains
KW - Subcellular Fractions
KW - Temperature
M3 - Journal article
C2 - 2065081
VL - 1074
SP - 284
EP - 288
JO - B B A - General Subjects
JF - B B A - General Subjects
SN - 0304-4165
IS - 2
ER -
ID: 293336