The evolutionarily conserved choroid plexus contributes to the homeostasis of brain ventricles in zebrafish

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The choroid plexus (ChP) produces cerebrospinal fluid (CSF). It also contributes to brain development and serves as the CSF-blood barrier. Prior studies have identified transporters on the epithelial cells that transport water and ions from the blood vasculature to the ventricles and tight junctions involved in the CSF-blood barrier. Yet, how the ChP epithelial cells control brain physiology remains unresolved. We use zebrafish to provide insights into the physiological roles of the ChP. Upon histological and transcriptomic analyses, we identify that the zebrafish ChP is conserved with mammals and expresses transporters involved in CSF secretion. Next, we show that the ChP epithelial cells secrete proteins into CSF. By ablating the ChP epithelial cells, we identify a reduction of the ventricular sizes without alterations of the CSF-blood barrier. Altogether, our findings reveal that the zebrafish ChP is conserved and contributes to the size and homeostasis of the brain ventricles.
OriginalsprogEngelsk
Artikelnummer114331
TidsskriftCell Reports
Vol/bind43
Udgave nummer6
Antal sider24
ISSN2211-1247
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
We thank the Fluorescent Reporter Zebrafish Cooperation Center (FRZCC), Republic of Korea, for the FRZCC1104 line; Val\u00E9rie Wittamer for the 4C4 antibody; Sebastian Gerety for the pUAS-Self vector; and the fish facility staff and all members of the Jurisch-Yaksi/Yaksi laboratories for their feedback. BioRender was used for illustrations. This work was supported by The Research Council of Norway ( 314189 ) to N.J.-Y.; Deutsche Forschungsgemeinschaft (DFG, Germany Research Foundation), FOR5547 - Project-ID 503306912 to N.J.-Y.; a National Research Foundation of Korea grant funded by the Korea government (MSIT) ( 2022R1A2C1091336 ) to H.-C.P.; the Lundbeck Foundation (to N.M.; R276-2018-403 ); the Fondation ARC pour la Recherche sur le Cancer ( PJA2018208167 to M.F., A2011 Postdoctoral fellowship to M.P.); the Human Frontiers Science Program ( CDA00036/2010 to M.F.); and the Cancerop\u00F4le PACA (Emergence 2024 to M.F.).

Funding Information:
We thank the Fluorescent Reporter Zebrafish Cooperation Center (FRZCC), Republic of Korea, for the FRZCC1104 line; Val\u00E9rie Wittamer for the 4C4 antibody; Sebastian Gerety for the pUAS-Self vector; Jakob M\u00F8rkved Stenersen for the initial nitroreductase optimization; and the fish facility staff and all members of the Jurisch-Yaksi/Yaksi laboratories for their feedback. BioRender was used for illustrations. This work was supported by The Research Council of Norway (314189) to N.J.-Y.; Deutsche Forschungsgemeinschaft (DFG, Germany Research Foundation), FOR5547 - Project-ID 503306912 to N.J.-Y.; a National Research Foundation of Korea grant funded by the Korea government (MSIT) (2022R1A2C1091336) to H.-C.P.; the Lundbeck Foundation (to N.M.; R276-2018-403); the Fondation ARC pour la Recherche sur le Cancer (PJA2018208167 to M.F. A2011 Postdoctoral fellowship to M.P.); the Human Frontiers Science Program (CDA00036/2010 to M.F.); and the Cancerop\u00F4le PACA (Emergence 2024 to M.F.). Conceptualization, N.J.-Y. I.J. N.M. S.N.A. and M.F.; methodology, I.J. S.N.A. L.H. M.P. Y.S. and M.F.; formal analysis, I.J. N.J.-Y. and S.N.A.; investigation, I.J. S.N.A. N.J.-Y. and L.H.; resources, N.J.-Y. H.-C.P. M.F. and N.M.; data curation, I.J. N.J.-Y. and S.N.A.; writing \u2013 original draft, I.J. and N.J.-Y.; writing \u2013 review & editing, all authors; visualization, I.J. N.J.-Y. and S.N.A.; supervision, N.J.-Y. H.-C.P. N.M. and M.F.; funding acquisition, N.J.-Y. H.-C.P. N.M. M.F. and M.P. The authors declare no competing interests.

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© 2024 The Authors

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