Studies on the native forms of renin in the rat kidney
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Studies on the native forms of renin in the rat kidney. / Rubin, Inger; Lauritzen, Edgar; Lauritzen, Martin.
I: BBA - Enzymology, Bind 612, Nr. 1, 14.03.1980, s. 126-136.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Studies on the native forms of renin in the rat kidney
AU - Rubin, Inger
AU - Lauritzen, Edgar
AU - Lauritzen, Martin
PY - 1980/3/14
Y1 - 1980/3/14
N2 - This paper describes Sephadex G-100 chromatography of rat kidney extract containing various enzyme inhibitors. The high molecular weight renin (molecular weight above 50 000) constitutes about 50% of the total renin activity. Omission of the enzyme inhibitors yield solely low molecular weight renin. Upon rechromatography high molecular weight renin eluted in two peaks at lower molecular weight with a concomitant reduction of renin activity. Renin activity in the fractions from Sephadex G-100 chromatography was increased 70% by dialysis at acid as well as neutral pH through the whole molecular weight range. Cold storage of extract with low molecular weight increased renin activity about 25%. The results suggest that the fully active enzyme is not represented by the lower molecular weight forms of renin and direct connection between activation of renin and reduction of renin molecular size was not indicated.
AB - This paper describes Sephadex G-100 chromatography of rat kidney extract containing various enzyme inhibitors. The high molecular weight renin (molecular weight above 50 000) constitutes about 50% of the total renin activity. Omission of the enzyme inhibitors yield solely low molecular weight renin. Upon rechromatography high molecular weight renin eluted in two peaks at lower molecular weight with a concomitant reduction of renin activity. Renin activity in the fractions from Sephadex G-100 chromatography was increased 70% by dialysis at acid as well as neutral pH through the whole molecular weight range. Cold storage of extract with low molecular weight increased renin activity about 25%. The results suggest that the fully active enzyme is not represented by the lower molecular weight forms of renin and direct connection between activation of renin and reduction of renin molecular size was not indicated.
KW - (Rat kidney)
KW - Molecular forms
KW - Renin
UR - http://www.scopus.com/inward/record.url?scp=0019152694&partnerID=8YFLogxK
U2 - 10.1016/0005-2744(80)90285-5
DO - 10.1016/0005-2744(80)90285-5
M3 - Journal article
C2 - 6988009
AN - SCOPUS:0019152694
VL - 612
SP - 126
EP - 136
JO - BBA - Enzymology
JF - BBA - Enzymology
SN - 0924-1086
IS - 1
ER -
ID: 201460870