Single-copy insertion of transgenes in Caenorhabditis elegans.

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Standard

Single-copy insertion of transgenes in Caenorhabditis elegans. / Frøkjaer-Jensen, Christian; Davis, M Wayne; Hopkins, Christopher E; Newman, Blake J; Thummel, Jason M; Olesen, Søren-Peter; Grunnet, Morten; Jorgensen, Erik M.

I: Nature Genetics, Bind 40, Nr. 11, 2008, s. 1375-83.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Frøkjaer-Jensen, C, Davis, MW, Hopkins, CE, Newman, BJ, Thummel, JM, Olesen, S-P, Grunnet, M & Jorgensen, EM 2008, 'Single-copy insertion of transgenes in Caenorhabditis elegans.', Nature Genetics, bind 40, nr. 11, s. 1375-83. https://doi.org/10.1038/ng.248

APA

Frøkjaer-Jensen, C., Davis, M. W., Hopkins, C. E., Newman, B. J., Thummel, J. M., Olesen, S-P., Grunnet, M., & Jorgensen, E. M. (2008). Single-copy insertion of transgenes in Caenorhabditis elegans. Nature Genetics, 40(11), 1375-83. https://doi.org/10.1038/ng.248

Vancouver

Frøkjaer-Jensen C, Davis MW, Hopkins CE, Newman BJ, Thummel JM, Olesen S-P o.a. Single-copy insertion of transgenes in Caenorhabditis elegans. Nature Genetics. 2008;40(11):1375-83. https://doi.org/10.1038/ng.248

Author

Frøkjaer-Jensen, Christian ; Davis, M Wayne ; Hopkins, Christopher E ; Newman, Blake J ; Thummel, Jason M ; Olesen, Søren-Peter ; Grunnet, Morten ; Jorgensen, Erik M. / Single-copy insertion of transgenes in Caenorhabditis elegans. I: Nature Genetics. 2008 ; Bind 40, Nr. 11. s. 1375-83.

Bibtex

@article{2907df80ab5311ddb5e9000ea68e967b,
title = "Single-copy insertion of transgenes in Caenorhabditis elegans.",
abstract = "At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.",
author = "Christian Fr{\o}kjaer-Jensen and Davis, {M Wayne} and Hopkins, {Christopher E} and Newman, {Blake J} and Thummel, {Jason M} and S{\o}ren-Peter Olesen and Morten Grunnet and Jorgensen, {Erik M}",
year = "2008",
doi = "10.1038/ng.248",
language = "English",
volume = "40",
pages = "1375--83",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - Single-copy insertion of transgenes in Caenorhabditis elegans.

AU - Frøkjaer-Jensen, Christian

AU - Davis, M Wayne

AU - Hopkins, Christopher E

AU - Newman, Blake J

AU - Thummel, Jason M

AU - Olesen, Søren-Peter

AU - Grunnet, Morten

AU - Jorgensen, Erik M

PY - 2008

Y1 - 2008

N2 - At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.

AB - At present, transgenes in Caenorhabditis elegans are generated by injecting DNA into the germline. The DNA assembles into a semistable extrachromosomal array composed of many copies of injected DNA. These transgenes are typically overexpressed in somatic cells and silenced in the germline. We have developed a method that inserts a single copy of a transgene into a defined site. Mobilization of a Mos1 transposon generates a double-strand break in noncoding DNA. The break is repaired by copying DNA from an extrachromosomal template into the chromosomal site. Homozygous single-copy insertions can be obtained in less than 2 weeks by injecting approximately 20 worms. We have successfully inserted transgenes as long as 9 kb and verified that single copies are inserted at the targeted site. Single-copy transgenes are expressed at endogenous levels and can be expressed in the female and male germlines.

U2 - 10.1038/ng.248

DO - 10.1038/ng.248

M3 - Journal article

C2 - 18953339

VL - 40

SP - 1375

EP - 1383

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 11

ER -

ID: 8418416