Regulation of T cell receptor-alpha gene recombination by transcription.

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Regulation of T cell receptor-alpha gene recombination by transcription. / Abarrategui, Iratxe; Krangel, Michael S.

I: Nature Immunology, Bind 7, Nr. 10, 2006, s. 1109-15.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Abarrategui, I & Krangel, MS 2006, 'Regulation of T cell receptor-alpha gene recombination by transcription.', Nature Immunology, bind 7, nr. 10, s. 1109-15. https://doi.org/10.1038/ni1379

APA

Abarrategui, I., & Krangel, M. S. (2006). Regulation of T cell receptor-alpha gene recombination by transcription. Nature Immunology, 7(10), 1109-15. https://doi.org/10.1038/ni1379

Vancouver

Abarrategui I, Krangel MS. Regulation of T cell receptor-alpha gene recombination by transcription. Nature Immunology. 2006;7(10):1109-15. https://doi.org/10.1038/ni1379

Author

Abarrategui, Iratxe ; Krangel, Michael S. / Regulation of T cell receptor-alpha gene recombination by transcription. I: Nature Immunology. 2006 ; Bind 7, Nr. 10. s. 1109-15.

Bibtex

@article{0d466f005ca211dd8d9f000ea68e967b,
title = "Regulation of T cell receptor-alpha gene recombination by transcription.",
abstract = "Despite the longstanding correlation between transcription and variable-(diversity)-joining (V(D)J) recombination, it is unknown whether transcription itself can direct recombinase targeting. Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. Transcriptional blockade also derepressed cryptic J(alpha) promoters. Our results demonstrate two key functions for transcription in Tcra locus regulation. Transcription increases the recombination of J(alpha) segments located within several kilobases of a promoter and prevents the activation of downstream promoters through transcriptional interference. These influences promote an ordered progression of Tcra locus recombination events and selection of a robust Tcra repertoire.",
author = "Iratxe Abarrategui and Krangel, {Michael S}",
note = "Keywords: Animals; Chromatin Assembly and Disassembly; Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; Genes, T-Cell Receptor alpha; Immunoglobulin Joining Region; Mice; Mice, Mutant Strains; Promoter Regions (Genetics); Recombination, Genetic; Sequence Deletion; Transcription, Genetic",
year = "2006",
doi = "10.1038/ni1379",
language = "English",
volume = "7",
pages = "1109--15",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "nature publishing group",
number = "10",

}

RIS

TY - JOUR

T1 - Regulation of T cell receptor-alpha gene recombination by transcription.

AU - Abarrategui, Iratxe

AU - Krangel, Michael S

N1 - Keywords: Animals; Chromatin Assembly and Disassembly; Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor; Genes, T-Cell Receptor alpha; Immunoglobulin Joining Region; Mice; Mice, Mutant Strains; Promoter Regions (Genetics); Recombination, Genetic; Sequence Deletion; Transcription, Genetic

PY - 2006

Y1 - 2006

N2 - Despite the longstanding correlation between transcription and variable-(diversity)-joining (V(D)J) recombination, it is unknown whether transcription itself can direct recombinase targeting. Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. Transcriptional blockade also derepressed cryptic J(alpha) promoters. Our results demonstrate two key functions for transcription in Tcra locus regulation. Transcription increases the recombination of J(alpha) segments located within several kilobases of a promoter and prevents the activation of downstream promoters through transcriptional interference. These influences promote an ordered progression of Tcra locus recombination events and selection of a robust Tcra repertoire.

AB - Despite the longstanding correlation between transcription and variable-(diversity)-joining (V(D)J) recombination, it is unknown whether transcription itself can direct recombinase targeting. Here we show that blockade of transcriptional elongation through the mouse T cell receptor-alpha (Tcra) locus suppressed V(alpha)-to-J(alpha) recombination and chromatin remodeling of J(alpha) segments. Transcriptional blockade also derepressed cryptic J(alpha) promoters. Our results demonstrate two key functions for transcription in Tcra locus regulation. Transcription increases the recombination of J(alpha) segments located within several kilobases of a promoter and prevents the activation of downstream promoters through transcriptional interference. These influences promote an ordered progression of Tcra locus recombination events and selection of a robust Tcra repertoire.

U2 - 10.1038/ni1379

DO - 10.1038/ni1379

M3 - Journal article

C2 - 16936730

VL - 7

SP - 1109

EP - 1115

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 10

ER -

ID: 5238065