Psoralen photofootprinting of protein-binding sites on DNA

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Standard

Psoralen photofootprinting of protein-binding sites on DNA. / Zhen, W P; Jeppesen, Claus; Nielsen, Peter E.

I: FEBS Letters, Bind 229, Nr. 1, 29.02.1988, s. 73-6.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Zhen, WP, Jeppesen, C & Nielsen, PE 1988, 'Psoralen photofootprinting of protein-binding sites on DNA', FEBS Letters, bind 229, nr. 1, s. 73-6. https://doi.org/10.1016/0014-5793(88)80800-7

APA

Zhen, W. P., Jeppesen, C., & Nielsen, P. E. (1988). Psoralen photofootprinting of protein-binding sites on DNA. FEBS Letters, 229(1), 73-6. https://doi.org/10.1016/0014-5793(88)80800-7

Vancouver

Zhen WP, Jeppesen C, Nielsen PE. Psoralen photofootprinting of protein-binding sites on DNA. FEBS Letters. 1988 feb. 29;229(1):73-6. https://doi.org/10.1016/0014-5793(88)80800-7

Author

Zhen, W P ; Jeppesen, Claus ; Nielsen, Peter E. / Psoralen photofootprinting of protein-binding sites on DNA. I: FEBS Letters. 1988 ; Bind 229, Nr. 1. s. 73-6.

Bibtex

@article{067ad4d0a8194473938fdc92b971ed0b,
title = "Psoralen photofootprinting of protein-binding sites on DNA",
abstract = "Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.",
keywords = "Animals, Base Sequence, Binding Sites, Cattle, DNA/metabolism, DNA-Binding Proteins, DNA-Directed RNA Polymerases/metabolism, Ficusin/metabolism, Furocoumarins/metabolism, Molecular Sequence Data, Photochemistry, Protein Binding, Repressor Proteins/metabolism, Trioxsalen/metabolism, Viral Proteins, Viral Regulatory and Accessory Proteins",
author = "Zhen, {W P} and Claus Jeppesen and Nielsen, {Peter E.}",
year = "1988",
month = feb,
day = "29",
doi = "10.1016/0014-5793(88)80800-7",
language = "English",
volume = "229",
pages = "73--6",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Psoralen photofootprinting of protein-binding sites on DNA

AU - Zhen, W P

AU - Jeppesen, Claus

AU - Nielsen, Peter E.

PY - 1988/2/29

Y1 - 1988/2/29

N2 - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

AB - Using a BAL31 exonuclease assay to determine the sites of 4,5',8-trimethylpsoralen photocrosslinking in DNA we have shown that 5'-TA sites which are accessible to psoralen DNA interstrand photocrosslinking in naked DNA become inaccessible when protein, in casu, lambda-repressor E. coli or RNA polymerase are bound at their recognition DNA sequences (OR1 operator or deo1 promoter, respectively). These results show that psoralens can be used as photofootprinting reagents to study specific protein-DNA interactions.

KW - Animals

KW - Base Sequence

KW - Binding Sites

KW - Cattle

KW - DNA/metabolism

KW - DNA-Binding Proteins

KW - DNA-Directed RNA Polymerases/metabolism

KW - Ficusin/metabolism

KW - Furocoumarins/metabolism

KW - Molecular Sequence Data

KW - Photochemistry

KW - Protein Binding

KW - Repressor Proteins/metabolism

KW - Trioxsalen/metabolism

KW - Viral Proteins

KW - Viral Regulatory and Accessory Proteins

U2 - 10.1016/0014-5793(88)80800-7

DO - 10.1016/0014-5793(88)80800-7

M3 - Journal article

C2 - 3345840

VL - 229

SP - 73

EP - 76

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1

ER -

ID: 203632482