Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide

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Standard

Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide. / Kofod, Hans; Unson, C G; Merrifield, R B.

I: International Journal of Peptide and Protein Research, Bind 32, Nr. 6, 12.1988, s. 436-40.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Kofod, H, Unson, CG & Merrifield, RB 1988, 'Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide', International Journal of Peptide and Protein Research, bind 32, nr. 6, s. 436-40.

APA

Kofod, H., Unson, C. G., & Merrifield, R. B. (1988). Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide. International Journal of Peptide and Protein Research, 32(6), 436-40.

Vancouver

Kofod H, Unson CG, Merrifield RB. Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide. International Journal of Peptide and Protein Research. 1988 dec.;32(6):436-40.

Author

Kofod, Hans ; Unson, C G ; Merrifield, R B. / Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide. I: International Journal of Peptide and Protein Research. 1988 ; Bind 32, Nr. 6. s. 436-40.

Bibtex

@article{fb9cbf121b414532aff40d35ed7c99ad,
title = "Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide",
abstract = "Glucagon and secretin and some of their hybrid analogs potentiate glucose-induced release of insulin from isolated mouse pancreatic islets. It was recently shown that the synthetic glucagon analog, desHis1[Glu9]glucagon amide, does not stimulate the formation of cyclic adenosine monophosphate in the rat hepatocyte membrane, but binds well to the glucagon receptor and is a good competitive antagonist of glucagon. In the present study the effect of this analog on isolated islets was examined. desHis1-[Glu9]glucagon amide at 3 x 10(-7) M, in the presence of 0.01 M D-glucose, increased the release of insulin by 30% and maintained that level for the full 30-min test period. The rate of insulin release returned to the glucose-induced base line after removal of the peptide. The same insulin level was produced by 3 x 10(-9) M glucagon, and at 3 x 10(-7) M glucagon insulin release was enhanced 290% above the glucose base line.",
keywords = "Amino Acid Sequence, Animals, Drug Synergism, Glucagon, Glucose, Indicators and Reagents, Insulin, Islets of Langerhans, Kinetics, Male, Mice, Mice, Inbred Strains",
author = "Hans Kofod and Unson, {C G} and Merrifield, {R B}",
year = "1988",
month = dec,
language = "English",
volume = "32",
pages = "436--40",
journal = "International Journal of Peptide and Protein Research",
number = "6",

}

RIS

TY - JOUR

T1 - Potentiation of glucose-induced insulin release in islets by desHis1[Glu9]glucagon amide

AU - Kofod, Hans

AU - Unson, C G

AU - Merrifield, R B

PY - 1988/12

Y1 - 1988/12

N2 - Glucagon and secretin and some of their hybrid analogs potentiate glucose-induced release of insulin from isolated mouse pancreatic islets. It was recently shown that the synthetic glucagon analog, desHis1[Glu9]glucagon amide, does not stimulate the formation of cyclic adenosine monophosphate in the rat hepatocyte membrane, but binds well to the glucagon receptor and is a good competitive antagonist of glucagon. In the present study the effect of this analog on isolated islets was examined. desHis1-[Glu9]glucagon amide at 3 x 10(-7) M, in the presence of 0.01 M D-glucose, increased the release of insulin by 30% and maintained that level for the full 30-min test period. The rate of insulin release returned to the glucose-induced base line after removal of the peptide. The same insulin level was produced by 3 x 10(-9) M glucagon, and at 3 x 10(-7) M glucagon insulin release was enhanced 290% above the glucose base line.

AB - Glucagon and secretin and some of their hybrid analogs potentiate glucose-induced release of insulin from isolated mouse pancreatic islets. It was recently shown that the synthetic glucagon analog, desHis1[Glu9]glucagon amide, does not stimulate the formation of cyclic adenosine monophosphate in the rat hepatocyte membrane, but binds well to the glucagon receptor and is a good competitive antagonist of glucagon. In the present study the effect of this analog on isolated islets was examined. desHis1-[Glu9]glucagon amide at 3 x 10(-7) M, in the presence of 0.01 M D-glucose, increased the release of insulin by 30% and maintained that level for the full 30-min test period. The rate of insulin release returned to the glucose-induced base line after removal of the peptide. The same insulin level was produced by 3 x 10(-9) M glucagon, and at 3 x 10(-7) M glucagon insulin release was enhanced 290% above the glucose base line.

KW - Amino Acid Sequence

KW - Animals

KW - Drug Synergism

KW - Glucagon

KW - Glucose

KW - Indicators and Reagents

KW - Insulin

KW - Islets of Langerhans

KW - Kinetics

KW - Male

KW - Mice

KW - Mice, Inbred Strains

M3 - Journal article

C2 - 3073146

VL - 32

SP - 436

EP - 440

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

IS - 6

ER -

ID: 45575036