Post-translational suppression of expression of intestinal brush border enzymes by fructose

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Standard

Post-translational suppression of expression of intestinal brush border enzymes by fructose. / Danielsen, E M.

I: Journal of Biological Chemistry, Bind 264, Nr. 23, 1989, s. 13726-9.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Danielsen, EM 1989, 'Post-translational suppression of expression of intestinal brush border enzymes by fructose', Journal of Biological Chemistry, bind 264, nr. 23, s. 13726-9.

APA

Danielsen, E. M. (1989). Post-translational suppression of expression of intestinal brush border enzymes by fructose. Journal of Biological Chemistry, 264(23), 13726-9.

Vancouver

Danielsen EM. Post-translational suppression of expression of intestinal brush border enzymes by fructose. Journal of Biological Chemistry. 1989;264(23):13726-9.

Author

Danielsen, E M. / Post-translational suppression of expression of intestinal brush border enzymes by fructose. I: Journal of Biological Chemistry. 1989 ; Bind 264, Nr. 23. s. 13726-9.

Bibtex

@article{a2e97660e7bc11ddbf70000ea68e967b,
title = "Post-translational suppression of expression of intestinal brush border enzymes by fructose",
abstract = "The two major dietary sugars, fructose and sucrose, were found to suppress effectively the biosynthetic renewal of brush border enzymes in the gut. When studied in cultured explants of pig small intestine mucosa, 10-50 mM concentrations of fructose completely prevented the expression of mature aminopeptidase N and severely reduced that of sucrase-isomaltase. The instantly occurring and reversible suppressive effect manifested itself as a leupeptin-sensitive degradation of newly synthesized brush border enzymes. The likely mechanism of action of the dietary sugar is by causing an abnormal cotranslational glycosylation that in turn triggers a rapid proteolytic breakdown. Our findings suggest that renewal of digestive brush border enzymes is transiently suppressed during intake of fructose- or sucrose-rich meals.",
author = "Danielsen, {E M}",
note = "Keywords: Aminopeptidases; Animals; Antigens, CD13; Enzyme Repression; Fructose; Intestinal Mucosa; Jejunum; Kinetics; Microvilli; Molecular Weight; Multienzyme Complexes; Organ Culture Techniques; Protein Processing, Post-Translational; Sucrase-Isomaltase Complex; Swine",
year = "1989",
language = "English",
volume = "264",
pages = "13726--9",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "23",

}

RIS

TY - JOUR

T1 - Post-translational suppression of expression of intestinal brush border enzymes by fructose

AU - Danielsen, E M

N1 - Keywords: Aminopeptidases; Animals; Antigens, CD13; Enzyme Repression; Fructose; Intestinal Mucosa; Jejunum; Kinetics; Microvilli; Molecular Weight; Multienzyme Complexes; Organ Culture Techniques; Protein Processing, Post-Translational; Sucrase-Isomaltase Complex; Swine

PY - 1989

Y1 - 1989

N2 - The two major dietary sugars, fructose and sucrose, were found to suppress effectively the biosynthetic renewal of brush border enzymes in the gut. When studied in cultured explants of pig small intestine mucosa, 10-50 mM concentrations of fructose completely prevented the expression of mature aminopeptidase N and severely reduced that of sucrase-isomaltase. The instantly occurring and reversible suppressive effect manifested itself as a leupeptin-sensitive degradation of newly synthesized brush border enzymes. The likely mechanism of action of the dietary sugar is by causing an abnormal cotranslational glycosylation that in turn triggers a rapid proteolytic breakdown. Our findings suggest that renewal of digestive brush border enzymes is transiently suppressed during intake of fructose- or sucrose-rich meals.

AB - The two major dietary sugars, fructose and sucrose, were found to suppress effectively the biosynthetic renewal of brush border enzymes in the gut. When studied in cultured explants of pig small intestine mucosa, 10-50 mM concentrations of fructose completely prevented the expression of mature aminopeptidase N and severely reduced that of sucrase-isomaltase. The instantly occurring and reversible suppressive effect manifested itself as a leupeptin-sensitive degradation of newly synthesized brush border enzymes. The likely mechanism of action of the dietary sugar is by causing an abnormal cotranslational glycosylation that in turn triggers a rapid proteolytic breakdown. Our findings suggest that renewal of digestive brush border enzymes is transiently suppressed during intake of fructose- or sucrose-rich meals.

M3 - Journal article

C2 - 2569463

VL - 264

SP - 13726

EP - 13729

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 23

ER -

ID: 9881016