Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives

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Standard

Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives. / Jeppesen, Claus; Nielsen, Peter E.

I: European Journal of Biochemistry, Bind 182, Nr. 2, 15.06.1989, s. 437-44.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Jeppesen, C & Nielsen, PE 1989, 'Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives', European Journal of Biochemistry, bind 182, nr. 2, s. 437-44. https://doi.org/10.1111/j.1432-1033.1989.tb14850.x

APA

Jeppesen, C., & Nielsen, P. E. (1989). Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives. European Journal of Biochemistry, 182(2), 437-44. https://doi.org/10.1111/j.1432-1033.1989.tb14850.x

Vancouver

Jeppesen C, Nielsen PE. Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives. European Journal of Biochemistry. 1989 jun. 15;182(2):437-44. https://doi.org/10.1111/j.1432-1033.1989.tb14850.x

Author

Jeppesen, Claus ; Nielsen, Peter E. / Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives. I: European Journal of Biochemistry. 1989 ; Bind 182, Nr. 2. s. 437-44.

Bibtex

@article{354b8c2edc7044f3900a4ac040ff0d48,
title = "Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives",
abstract = "It is demonstrated that DNA photofootprinting analysis of the intercalating depsipeptide echinomycin, and the minor groove-binders distamicyn, 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33258 can be performed using 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) [Nielsen et al. (1988) Nucleic Acids Res. 16, 3877-3888] or 2-methoxy-6-azido-9-aminoacridine (MAA) [Jeppesen et al. (1988) Nucleic Acids Res. 16, 5755-5770]. Both the extent of the drug-binding sites and their relative strength can be determined with either reagent. DNA has the advantage of giving virtually sequence-uniform DNA photocleavage. On the other hand, structural changes in the DNA are detected by MAA. Using the 232-base-pair EcoRI-PvuII pUC19 restriction fragment, it is found that cleavage protection by distamycin, DAPI and Hoechst 33258 all require an (A.T)4 sequence, whereas protection by echinomycin was confined to a G + C-rich 8-base-pair region.",
keywords = "Aminoacridines, Azides, Base Sequence, Bisbenzimidazole/analysis, DNA/analysis, DNA Damage, DNA Restriction Enzymes, Distamycins/analysis, Echinomycin/analysis, Indoles/analysis, Molecular Sequence Data, Molecular Structure, Plasmids, Receptors, Drug/analysis",
author = "Claus Jeppesen and Nielsen, {Peter E.}",
year = "1989",
month = jun,
day = "15",
doi = "10.1111/j.1432-1033.1989.tb14850.x",
language = "English",
volume = "182",
pages = "437--44",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Springer Verlag",
number = "2",

}

RIS

TY - JOUR

T1 - Photofootprinting of drug-binding sites on DNA using diazo- and azido-9-aminoacridine derivatives

AU - Jeppesen, Claus

AU - Nielsen, Peter E.

PY - 1989/6/15

Y1 - 1989/6/15

N2 - It is demonstrated that DNA photofootprinting analysis of the intercalating depsipeptide echinomycin, and the minor groove-binders distamicyn, 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33258 can be performed using 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) [Nielsen et al. (1988) Nucleic Acids Res. 16, 3877-3888] or 2-methoxy-6-azido-9-aminoacridine (MAA) [Jeppesen et al. (1988) Nucleic Acids Res. 16, 5755-5770]. Both the extent of the drug-binding sites and their relative strength can be determined with either reagent. DNA has the advantage of giving virtually sequence-uniform DNA photocleavage. On the other hand, structural changes in the DNA are detected by MAA. Using the 232-base-pair EcoRI-PvuII pUC19 restriction fragment, it is found that cleavage protection by distamycin, DAPI and Hoechst 33258 all require an (A.T)4 sequence, whereas protection by echinomycin was confined to a G + C-rich 8-base-pair region.

AB - It is demonstrated that DNA photofootprinting analysis of the intercalating depsipeptide echinomycin, and the minor groove-binders distamicyn, 4',6-diamidino-2-phenylindole (DAPI) and Hoechst 33258 can be performed using 9-[6-(2-diazocyclopentadienylcarbonyloxy)hexylamino]acridine (DHA) [Nielsen et al. (1988) Nucleic Acids Res. 16, 3877-3888] or 2-methoxy-6-azido-9-aminoacridine (MAA) [Jeppesen et al. (1988) Nucleic Acids Res. 16, 5755-5770]. Both the extent of the drug-binding sites and their relative strength can be determined with either reagent. DNA has the advantage of giving virtually sequence-uniform DNA photocleavage. On the other hand, structural changes in the DNA are detected by MAA. Using the 232-base-pair EcoRI-PvuII pUC19 restriction fragment, it is found that cleavage protection by distamycin, DAPI and Hoechst 33258 all require an (A.T)4 sequence, whereas protection by echinomycin was confined to a G + C-rich 8-base-pair region.

KW - Aminoacridines

KW - Azides

KW - Base Sequence

KW - Bisbenzimidazole/analysis

KW - DNA/analysis

KW - DNA Damage

KW - DNA Restriction Enzymes

KW - Distamycins/analysis

KW - Echinomycin/analysis

KW - Indoles/analysis

KW - Molecular Sequence Data

KW - Molecular Structure

KW - Plasmids

KW - Receptors, Drug/analysis

U2 - 10.1111/j.1432-1033.1989.tb14850.x

DO - 10.1111/j.1432-1033.1989.tb14850.x

M3 - Journal article

C2 - 2472274

VL - 182

SP - 437

EP - 444

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 2

ER -

ID: 203632259