Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine. / Nielsen, Peter E.; Jeppesen, C; Buchardt, O; Egholm, M.

I: Biochemistry, Bind 27, Nr. 17, 23.08.1988, s. 6338-43.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nielsen, PE, Jeppesen, C, Buchardt, O & Egholm, M 1988, 'Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine', Biochemistry, bind 27, nr. 17, s. 6338-43. https://doi.org/10.1021/bi00417a021

APA

Nielsen, P. E., Jeppesen, C., Buchardt, O., & Egholm, M. (1988). Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine. Biochemistry, 27(17), 6338-43. https://doi.org/10.1021/bi00417a021

Vancouver

Nielsen PE, Jeppesen C, Buchardt O, Egholm M. Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine. Biochemistry. 1988 aug. 23;27(17):6338-43. https://doi.org/10.1021/bi00417a021

Author

Nielsen, Peter E. ; Jeppesen, C ; Buchardt, O ; Egholm, M. / Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine. I: Biochemistry. 1988 ; Bind 27, Nr. 17. s. 6338-43.

Bibtex

@article{a39c9daba0954ce0919e3137c24e23cf,
title = "Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine",
abstract = "Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]amino]-acridine. Removal of the acridinyl ligand or changing the position of the nitro group from the 4- to the 2-position caused a 10-fold decrease in photocleavage efficiency, whereas a change to the 3-position caused a 30-fold reduction. The DNA cleavage was 5-fold enhanced by subsequent piperidine treatment and showed some sequence dependency with predominant cleavage at G and T residues. Furthermore, significant differences in cleavage preference were observed when the poly(methylene) linker length was changed.",
keywords = "Aminoacridines/chemical synthesis, Benzamides/pharmacology, DNA/drug effects, Indicators and Reagents, Nitro Compounds/pharmacology, Photolysis, Plasmids/drug effects, Structure-Activity Relationship",
author = "Nielsen, {Peter E.} and C Jeppesen and O Buchardt and M Egholm",
year = "1988",
month = aug,
day = "23",
doi = "10.1021/bi00417a021",
language = "English",
volume = "27",
pages = "6338--43",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "17",

}

RIS

TY - JOUR

T1 - Photochemical cleavage of DNA by nitrobenzamides linked to 9-aminoacridine

AU - Nielsen, Peter E.

AU - Jeppesen, C

AU - Buchardt, O

AU - Egholm, M

PY - 1988/8/23

Y1 - 1988/8/23

N2 - Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]amino]-acridine. Removal of the acridinyl ligand or changing the position of the nitro group from the 4- to the 2-position caused a 10-fold decrease in photocleavage efficiency, whereas a change to the 3-position caused a 30-fold reduction. The DNA cleavage was 5-fold enhanced by subsequent piperidine treatment and showed some sequence dependency with predominant cleavage at G and T residues. Furthermore, significant differences in cleavage preference were observed when the poly(methylene) linker length was changed.

AB - Nitrobenzamido ligands linked to the DNA intercalator 9-aminoacridine via poly(methylene) chains induce single-strand nicks in DNA upon irradiation with long-wavelength ultraviolet light (lambda greater than or equal to 300 nm). Optimal photocleavage activity was found for the reagent 9-[[6-(4-nitrobenzamido)hexyl]amino]-acridine. Removal of the acridinyl ligand or changing the position of the nitro group from the 4- to the 2-position caused a 10-fold decrease in photocleavage efficiency, whereas a change to the 3-position caused a 30-fold reduction. The DNA cleavage was 5-fold enhanced by subsequent piperidine treatment and showed some sequence dependency with predominant cleavage at G and T residues. Furthermore, significant differences in cleavage preference were observed when the poly(methylene) linker length was changed.

KW - Aminoacridines/chemical synthesis

KW - Benzamides/pharmacology

KW - DNA/drug effects

KW - Indicators and Reagents

KW - Nitro Compounds/pharmacology

KW - Photolysis

KW - Plasmids/drug effects

KW - Structure-Activity Relationship

U2 - 10.1021/bi00417a021

DO - 10.1021/bi00417a021

M3 - Journal article

C2 - 3219340

VL - 27

SP - 6338

EP - 6343

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 17

ER -

ID: 203631211