On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study

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Standard

On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study. / Zhen, W P; Dahl, O; Buchardt, O; Nielsen, Peter E.

I: Photochemistry and Photobiology, Bind 48, Nr. 5, 11.1988, s. 643-6.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Zhen, WP, Dahl, O, Buchardt, O & Nielsen, PE 1988, 'On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study', Photochemistry and Photobiology, bind 48, nr. 5, s. 643-6. https://doi.org/10.1111/j.1751-1097.1988.tb02875.x

APA

Zhen, W. P., Dahl, O., Buchardt, O., & Nielsen, P. E. (1988). On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study. Photochemistry and Photobiology, 48(5), 643-6. https://doi.org/10.1111/j.1751-1097.1988.tb02875.x

Vancouver

Zhen WP, Dahl O, Buchardt O, Nielsen PE. On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study. Photochemistry and Photobiology. 1988 nov.;48(5):643-6. https://doi.org/10.1111/j.1751-1097.1988.tb02875.x

Author

Zhen, W P ; Dahl, O ; Buchardt, O ; Nielsen, Peter E. / On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study. I: Photochemistry and Photobiology. 1988 ; Bind 48, Nr. 5. s. 643-6.

Bibtex

@article{b82a9ad5fd7341ea8ba20049b9583794,
title = "On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study",
abstract = "A synthetic, partially double stranded decadeoxyribonucleotide with cohesive ends, containing one potential psoralen photo‐crosslinking site centrally positioned (5′‐d(CGGGCTACCC) + 3′‐d(CCGATGGGGC)), has been ligated to double stranded DNA oligomers, which were subsequently photoreacted with 4,5′,8‐trimethylpsoralen. It was found that psoralen DNA interstrand crosslinking does not significantly alter the electrophoretic mobility of these DNA molecules in polyacrylamide gels. Based on this, we conclude that any bends in the DNA helix that may be induced by psoralen DNA interstrand crosslinking must be significantly less than the 45° proposed by Tomic et at. (1987) (Science, 238, 1722) and/or of a different nature than the DNA sequence dependent bends due to d(A)n tracts.",
keywords = "Cross-Linking Reagents, DNA, Furocoumarins, Oligodeoxyribonucleotides, Photochemistry, Trioxsalen",
author = "Zhen, {W P} and O Dahl and O Buchardt and Nielsen, {Peter E.}",
year = "1988",
month = nov,
doi = "10.1111/j.1751-1097.1988.tb02875.x",
language = "English",
volume = "48",
pages = "643--6",
journal = "Photochemistry and Photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - On the DNA bending by psoralen interstrand crosslinking. A gel electrophoretic study

AU - Zhen, W P

AU - Dahl, O

AU - Buchardt, O

AU - Nielsen, Peter E.

PY - 1988/11

Y1 - 1988/11

N2 - A synthetic, partially double stranded decadeoxyribonucleotide with cohesive ends, containing one potential psoralen photo‐crosslinking site centrally positioned (5′‐d(CGGGCTACCC) + 3′‐d(CCGATGGGGC)), has been ligated to double stranded DNA oligomers, which were subsequently photoreacted with 4,5′,8‐trimethylpsoralen. It was found that psoralen DNA interstrand crosslinking does not significantly alter the electrophoretic mobility of these DNA molecules in polyacrylamide gels. Based on this, we conclude that any bends in the DNA helix that may be induced by psoralen DNA interstrand crosslinking must be significantly less than the 45° proposed by Tomic et at. (1987) (Science, 238, 1722) and/or of a different nature than the DNA sequence dependent bends due to d(A)n tracts.

AB - A synthetic, partially double stranded decadeoxyribonucleotide with cohesive ends, containing one potential psoralen photo‐crosslinking site centrally positioned (5′‐d(CGGGCTACCC) + 3′‐d(CCGATGGGGC)), has been ligated to double stranded DNA oligomers, which were subsequently photoreacted with 4,5′,8‐trimethylpsoralen. It was found that psoralen DNA interstrand crosslinking does not significantly alter the electrophoretic mobility of these DNA molecules in polyacrylamide gels. Based on this, we conclude that any bends in the DNA helix that may be induced by psoralen DNA interstrand crosslinking must be significantly less than the 45° proposed by Tomic et at. (1987) (Science, 238, 1722) and/or of a different nature than the DNA sequence dependent bends due to d(A)n tracts.

KW - Cross-Linking Reagents

KW - DNA

KW - Furocoumarins

KW - Oligodeoxyribonucleotides

KW - Photochemistry

KW - Trioxsalen

U2 - 10.1111/j.1751-1097.1988.tb02875.x

DO - 10.1111/j.1751-1097.1988.tb02875.x

M3 - Journal article

C2 - 3241837

VL - 48

SP - 643

EP - 646

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

SN - 0031-8655

IS - 5

ER -

ID: 203631115