Nucleotide sequences of cDNA clones for B1 hordein polypeptides
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Nucleotide sequences of cDNA clones for B1 hordein polypeptides. / Rasmussen, Søren K.; Hopp, H. Esteban; Brandt, Anders.
I: Carlsberg Research Communications, Bind 48, Nr. 3, 01.05.1983, s. 187-199.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Nucleotide sequences of cDNA clones for B1 hordein polypeptides
AU - Rasmussen, Søren K.
AU - Hopp, H. Esteban
AU - Brandt, Anders
PY - 1983/5/1
Y1 - 1983/5/1
N2 - Nucleotide sequences of four cDNA clones coding for the carboxy-terminal portion of at least two different B1 hordein polypeptides are presented. The open reading frame in the nucleotide sequence of the the largest clone (pc hor2-4, 720 nucleotides) translates into the 181 carboxy-terminal amino acids of a polypeptide chain showing close homology to the previously determined primary structure of B1 hordein peptides. Of the 74 amino acid residues which can be compared 61 proved to be identical. The second cDNA clone (pc hor2-3, 257 nucleotides) encodes the 54 carboxy-terminal amino acids of a different B1 hordein polypeptide, which is revealed by 21 nucleotide substitutions resulting in 9 amino acid changes. The two other analysed cDNA clones contained sequences of 54 and 41 nucleotides respectively for the carboxy-terminal end of the same B1 hordein polypeptide as that coded for by pc hor2-3. The latter two clones (pc hor2-1, 254 nucleotides and pc hor2-2, 153 nucleotides) comparised the entire 3′ noncoding region of the B1 hordein messenger RNA including a poly(A) tail. Clone pc hor2-2 measures 99 nucleotides between between the stop codon and the poly(A) tail. A putative polyadenylation signal AATAAA is located 15 residues upstream from the poly(A) site. These 99 nucleotides of the 3′ noncoding region are extended by a sequence of 63 nucleotides in clone pc hor2-1. This additional sequence contains two AATAAA sequences located 13 and 24 nucleotides respectively from its poly(A) tail.
AB - Nucleotide sequences of four cDNA clones coding for the carboxy-terminal portion of at least two different B1 hordein polypeptides are presented. The open reading frame in the nucleotide sequence of the the largest clone (pc hor2-4, 720 nucleotides) translates into the 181 carboxy-terminal amino acids of a polypeptide chain showing close homology to the previously determined primary structure of B1 hordein peptides. Of the 74 amino acid residues which can be compared 61 proved to be identical. The second cDNA clone (pc hor2-3, 257 nucleotides) encodes the 54 carboxy-terminal amino acids of a different B1 hordein polypeptide, which is revealed by 21 nucleotide substitutions resulting in 9 amino acid changes. The two other analysed cDNA clones contained sequences of 54 and 41 nucleotides respectively for the carboxy-terminal end of the same B1 hordein polypeptide as that coded for by pc hor2-3. The latter two clones (pc hor2-1, 254 nucleotides and pc hor2-2, 153 nucleotides) comparised the entire 3′ noncoding region of the B1 hordein messenger RNA including a poly(A) tail. Clone pc hor2-2 measures 99 nucleotides between between the stop codon and the poly(A) tail. A putative polyadenylation signal AATAAA is located 15 residues upstream from the poly(A) site. These 99 nucleotides of the 3′ noncoding region are extended by a sequence of 63 nucleotides in clone pc hor2-1. This additional sequence contains two AATAAA sequences located 13 and 24 nucleotides respectively from its poly(A) tail.
KW - amino acid sequence
KW - Barley
KW - polyadenylation signal
KW - prolamin
KW - storage protein
UR - http://www.scopus.com/inward/record.url?scp=34250831887&partnerID=8YFLogxK
U2 - 10.1007/BF02907766
DO - 10.1007/BF02907766
M3 - Journal article
AN - SCOPUS:34250831887
VL - 48
SP - 187
EP - 199
JO - Carlsberg Research Communications
JF - Carlsberg Research Communications
SN - 0105-1938
IS - 3
ER -
ID: 204472555