Neuronal pH regulation: constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Neuronal pH regulation : constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study. / Vorstrup, S; Jensen, K E; Thomsen, C; Henriksen, O; Lassen, N A; Paulson, O B.

I: Journal of Cerebral Blood Flow and Metabolism, Bind 9, Nr. 3, 06.1989, s. 417-21.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vorstrup, S, Jensen, KE, Thomsen, C, Henriksen, O, Lassen, NA & Paulson, OB 1989, 'Neuronal pH regulation: constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study', Journal of Cerebral Blood Flow and Metabolism, bind 9, nr. 3, s. 417-21. https://doi.org/10.1038/jcbfm.1989.61

APA

Vorstrup, S., Jensen, K. E., Thomsen, C., Henriksen, O., Lassen, N. A., & Paulson, O. B. (1989). Neuronal pH regulation: constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study. Journal of Cerebral Blood Flow and Metabolism, 9(3), 417-21. https://doi.org/10.1038/jcbfm.1989.61

Vancouver

Vorstrup S, Jensen KE, Thomsen C, Henriksen O, Lassen NA, Paulson OB. Neuronal pH regulation: constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study. Journal of Cerebral Blood Flow and Metabolism. 1989 jun.;9(3):417-21. https://doi.org/10.1038/jcbfm.1989.61

Author

Vorstrup, S ; Jensen, K E ; Thomsen, C ; Henriksen, O ; Lassen, N A ; Paulson, O B. / Neuronal pH regulation : constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study. I: Journal of Cerebral Blood Flow and Metabolism. 1989 ; Bind 9, Nr. 3. s. 417-21.

Bibtex

@article{78ead97ce2c0495f8e0abc5d6830b830,
title = "Neuronal pH regulation: constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study",
abstract = "The intracellular pH in the brain was studied in six healthy volunteers before and immediately after the administration of 2 g of acetazolamide. Phosphorus-31 nuclear magnetic resonance spectroscopy by a 1.5 tesla whole-body scanner was used. The chemical shift between the inorganic phosphate and the phosphocreatine resonance frequencies was used for indirect assessment of the intracellular pH. The mean baseline intracellular pH was 7.05 +/- 0.04 (SD). The mean pH changes obtained at 15-min intervals within the first hour of acetazolamide administration were -0.03 +/- 0.04 (SD), -0.02 +/- 0.03 (SD), and 0.00 +/- 0.04 (SD), i.e., no statistically significant pH decrease was observed during the period where extracellular pH is known to drop markedly. Although several factors contribute to the lack of change of the intraneuronal pH, we will discuss that this observation in addition might suggest a direct intracerebral effect of acetazolamide.",
keywords = "Acetazolamide/pharmacology, Adenosine Triphosphate/metabolism, Adult, Brain/drug effects, Extracellular Space/metabolism, Female, Humans, Hydrogen-Ion Concentration, Intracellular Fluid/metabolism, Magnetic Resonance Spectroscopy, Male, Middle Aged, Neurons/metabolism, Phosphates/metabolism, Phosphocreatine/metabolism",
author = "S Vorstrup and Jensen, {K E} and C Thomsen and O Henriksen and Lassen, {N A} and Paulson, {O B}",
year = "1989",
month = jun,
doi = "10.1038/jcbfm.1989.61",
language = "English",
volume = "9",
pages = "417--21",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "SAGE Publications",
number = "3",

}

RIS

TY - JOUR

T1 - Neuronal pH regulation

T2 - constant normal intracellular pH is maintained in brain during low extracellular pH induced by acetazolamide--31P NMR study

AU - Vorstrup, S

AU - Jensen, K E

AU - Thomsen, C

AU - Henriksen, O

AU - Lassen, N A

AU - Paulson, O B

PY - 1989/6

Y1 - 1989/6

N2 - The intracellular pH in the brain was studied in six healthy volunteers before and immediately after the administration of 2 g of acetazolamide. Phosphorus-31 nuclear magnetic resonance spectroscopy by a 1.5 tesla whole-body scanner was used. The chemical shift between the inorganic phosphate and the phosphocreatine resonance frequencies was used for indirect assessment of the intracellular pH. The mean baseline intracellular pH was 7.05 +/- 0.04 (SD). The mean pH changes obtained at 15-min intervals within the first hour of acetazolamide administration were -0.03 +/- 0.04 (SD), -0.02 +/- 0.03 (SD), and 0.00 +/- 0.04 (SD), i.e., no statistically significant pH decrease was observed during the period where extracellular pH is known to drop markedly. Although several factors contribute to the lack of change of the intraneuronal pH, we will discuss that this observation in addition might suggest a direct intracerebral effect of acetazolamide.

AB - The intracellular pH in the brain was studied in six healthy volunteers before and immediately after the administration of 2 g of acetazolamide. Phosphorus-31 nuclear magnetic resonance spectroscopy by a 1.5 tesla whole-body scanner was used. The chemical shift between the inorganic phosphate and the phosphocreatine resonance frequencies was used for indirect assessment of the intracellular pH. The mean baseline intracellular pH was 7.05 +/- 0.04 (SD). The mean pH changes obtained at 15-min intervals within the first hour of acetazolamide administration were -0.03 +/- 0.04 (SD), -0.02 +/- 0.03 (SD), and 0.00 +/- 0.04 (SD), i.e., no statistically significant pH decrease was observed during the period where extracellular pH is known to drop markedly. Although several factors contribute to the lack of change of the intraneuronal pH, we will discuss that this observation in addition might suggest a direct intracerebral effect of acetazolamide.

KW - Acetazolamide/pharmacology

KW - Adenosine Triphosphate/metabolism

KW - Adult

KW - Brain/drug effects

KW - Extracellular Space/metabolism

KW - Female

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Intracellular Fluid/metabolism

KW - Magnetic Resonance Spectroscopy

KW - Male

KW - Middle Aged

KW - Neurons/metabolism

KW - Phosphates/metabolism

KW - Phosphocreatine/metabolism

U2 - 10.1038/jcbfm.1989.61

DO - 10.1038/jcbfm.1989.61

M3 - Journal article

C2 - 2715211

VL - 9

SP - 417

EP - 421

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 3

ER -

ID: 275592287