Nebulization and selective deposition of LTD4 in human lungs

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Nebulization and selective deposition of LTD4 in human lungs. / Bisgaard, H.; Poulsen, L.; Søndergaard, I.

I: Allergy, Bind 42, Nr. 5, 07.1987, s. 336-342.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bisgaard, H, Poulsen, L & Søndergaard, I 1987, 'Nebulization and selective deposition of LTD4 in human lungs', Allergy, bind 42, nr. 5, s. 336-342. https://doi.org/10.1111/j.1398-9995.1987.tb02219.x

APA

Bisgaard, H., Poulsen, L., & Søndergaard, I. (1987). Nebulization and selective deposition of LTD4 in human lungs. Allergy, 42(5), 336-342. https://doi.org/10.1111/j.1398-9995.1987.tb02219.x

Vancouver

Bisgaard H, Poulsen L, Søndergaard I. Nebulization and selective deposition of LTD4 in human lungs. Allergy. 1987 jul.;42(5):336-342. https://doi.org/10.1111/j.1398-9995.1987.tb02219.x

Author

Bisgaard, H. ; Poulsen, L. ; Søndergaard, I. / Nebulization and selective deposition of LTD4 in human lungs. I: Allergy. 1987 ; Bind 42, Nr. 5. s. 336-342.

Bibtex

@article{f9b1b7272b734732978edb63ee5d4a87,
title = "Nebulization and selective deposition of LTD4 in human lungs",
abstract = "The process of nebulization and deposition of LTD4 was studied in detail. The concentration of LTD4 in a saline solution decreased by approximately 90 % after 2 min of nebulization in a DeVilbiss 35B ultrasonic nebulizer. This decrease was prevented by diluting LTD4 in a phosphate buffer, pH 7.4. Nebulization of tritiated LTD4 in this phosphate buffer did not cause any appreciable deterioration of the leukotriene, as demonstrated by an unchanged ratio between radioactivity and LTD4 concentration in the test solution before and after nebulization as well as in the condensed aerosol. The aerosol generated by the DeVilbiss 35B ultrasonic nebulizer was shown to generate particles with a mass median diameter of 1.3 microns (dry particle size). Interposition of a settling bag reduced the amount of large particles, reducing the mass median diameter to 0.84 microns (dry particle size). Nine healthy volunteers were challenged on separate days with 40 nmol LTD4 or 100 nmol histamine, and the changes in FEV, and partial flow volume curves initiated at 50% of vital capacity (Vmax30) were measured. A relative diffuse deposition pattern was ensured by inhalation via a settling bag. These results were compared to challenges with a relatively central deposition pattern as ensured by inhalation directly from the nebulizer with brisk inhalation maneuvers. The diffuse deposition pattern caused minimal changes in FEV, but pronounced effect in Vmax30. The effects of LTD4 and histamine on FEV, and Vmax30 changed in parallel when the deposition of the mediators was changed to a more central pattern. This indicates that the two mediators do not differ with respect to any selective effects on different parts of the airways.",
keywords = "adsorption, bronchoconstriction, challenge, LTD, nebulization",
author = "H. Bisgaard and L. Poulsen and I. S{\o}ndergaard",
year = "1987",
month = jul,
doi = "10.1111/j.1398-9995.1987.tb02219.x",
language = "English",
volume = "42",
pages = "336--342",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley Online",
number = "5",

}

RIS

TY - JOUR

T1 - Nebulization and selective deposition of LTD4 in human lungs

AU - Bisgaard, H.

AU - Poulsen, L.

AU - Søndergaard, I.

PY - 1987/7

Y1 - 1987/7

N2 - The process of nebulization and deposition of LTD4 was studied in detail. The concentration of LTD4 in a saline solution decreased by approximately 90 % after 2 min of nebulization in a DeVilbiss 35B ultrasonic nebulizer. This decrease was prevented by diluting LTD4 in a phosphate buffer, pH 7.4. Nebulization of tritiated LTD4 in this phosphate buffer did not cause any appreciable deterioration of the leukotriene, as demonstrated by an unchanged ratio between radioactivity and LTD4 concentration in the test solution before and after nebulization as well as in the condensed aerosol. The aerosol generated by the DeVilbiss 35B ultrasonic nebulizer was shown to generate particles with a mass median diameter of 1.3 microns (dry particle size). Interposition of a settling bag reduced the amount of large particles, reducing the mass median diameter to 0.84 microns (dry particle size). Nine healthy volunteers were challenged on separate days with 40 nmol LTD4 or 100 nmol histamine, and the changes in FEV, and partial flow volume curves initiated at 50% of vital capacity (Vmax30) were measured. A relative diffuse deposition pattern was ensured by inhalation via a settling bag. These results were compared to challenges with a relatively central deposition pattern as ensured by inhalation directly from the nebulizer with brisk inhalation maneuvers. The diffuse deposition pattern caused minimal changes in FEV, but pronounced effect in Vmax30. The effects of LTD4 and histamine on FEV, and Vmax30 changed in parallel when the deposition of the mediators was changed to a more central pattern. This indicates that the two mediators do not differ with respect to any selective effects on different parts of the airways.

AB - The process of nebulization and deposition of LTD4 was studied in detail. The concentration of LTD4 in a saline solution decreased by approximately 90 % after 2 min of nebulization in a DeVilbiss 35B ultrasonic nebulizer. This decrease was prevented by diluting LTD4 in a phosphate buffer, pH 7.4. Nebulization of tritiated LTD4 in this phosphate buffer did not cause any appreciable deterioration of the leukotriene, as demonstrated by an unchanged ratio between radioactivity and LTD4 concentration in the test solution before and after nebulization as well as in the condensed aerosol. The aerosol generated by the DeVilbiss 35B ultrasonic nebulizer was shown to generate particles with a mass median diameter of 1.3 microns (dry particle size). Interposition of a settling bag reduced the amount of large particles, reducing the mass median diameter to 0.84 microns (dry particle size). Nine healthy volunteers were challenged on separate days with 40 nmol LTD4 or 100 nmol histamine, and the changes in FEV, and partial flow volume curves initiated at 50% of vital capacity (Vmax30) were measured. A relative diffuse deposition pattern was ensured by inhalation via a settling bag. These results were compared to challenges with a relatively central deposition pattern as ensured by inhalation directly from the nebulizer with brisk inhalation maneuvers. The diffuse deposition pattern caused minimal changes in FEV, but pronounced effect in Vmax30. The effects of LTD4 and histamine on FEV, and Vmax30 changed in parallel when the deposition of the mediators was changed to a more central pattern. This indicates that the two mediators do not differ with respect to any selective effects on different parts of the airways.

KW - adsorption

KW - bronchoconstriction

KW - challenge

KW - LTD

KW - nebulization

UR - http://www.scopus.com/inward/record.url?scp=0023245056&partnerID=8YFLogxK

U2 - 10.1111/j.1398-9995.1987.tb02219.x

DO - 10.1111/j.1398-9995.1987.tb02219.x

M3 - Journal article

C2 - 3631461

AN - SCOPUS:0023245056

VL - 42

SP - 336

EP - 342

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 5

ER -

ID: 330548981