Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

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Standard

Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI. / Andreassen, Patrick Rosendahl; Fredberg, Sofie; Horan, Mattias; Knudsen, Maria Højberg; Jacobsen, Kirstine; Kjær, Andreas; Krogh, Thøger Jensen; Kronborg, Tina; Mattsson, Nicky Cordua; Schmidt, Sissel Ida; Wille, Heidi; Andersen, Ann.

I: A C S Synthetic Biology, Bind 3, Nr. 12, 19.12.2014, s. 949-52.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Andreassen, PR, Fredberg, S, Horan, M, Knudsen, MH, Jacobsen, K, Kjær, A, Krogh, TJ, Kronborg, T, Mattsson, NC, Schmidt, SI, Wille, H & Andersen, A 2014, 'Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI', A C S Synthetic Biology, bind 3, nr. 12, s. 949-52. https://doi.org/10.1021/sb500031h

APA

Andreassen, P. R., Fredberg, S., Horan, M., Knudsen, M. H., Jacobsen, K., Kjær, A., Krogh, T. J., Kronborg, T., Mattsson, N. C., Schmidt, S. I., Wille, H., & Andersen, A. (2014). Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI. A C S Synthetic Biology, 3(12), 949-52. https://doi.org/10.1021/sb500031h

Vancouver

Andreassen PR, Fredberg S, Horan M, Knudsen MH, Jacobsen K, Kjær A o.a. Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI. A C S Synthetic Biology. 2014 dec. 19;3(12):949-52. https://doi.org/10.1021/sb500031h

Author

Andreassen, Patrick Rosendahl ; Fredberg, Sofie ; Horan, Mattias ; Knudsen, Maria Højberg ; Jacobsen, Kirstine ; Kjær, Andreas ; Krogh, Thøger Jensen ; Kronborg, Tina ; Mattsson, Nicky Cordua ; Schmidt, Sissel Ida ; Wille, Heidi ; Andersen, Ann. / Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI. I: A C S Synthetic Biology. 2014 ; Bind 3, Nr. 12. s. 949-52.

Bibtex

@article{4c0556b8a0f841e88ac9d8157d8595a6,
title = "Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI",
abstract = "The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.",
author = "Andreassen, {Patrick Rosendahl} and Sofie Fredberg and Mattias Horan and Knudsen, {Maria H{\o}jberg} and Kirstine Jacobsen and Andreas Kj{\ae}r and Krogh, {Th{\o}ger Jensen} and Tina Kronborg and Mattsson, {Nicky Cordua} and Schmidt, {Sissel Ida} and Heidi Wille and Ann Andersen",
year = "2014",
month = dec,
day = "19",
doi = "10.1021/sb500031h",
language = "English",
volume = "3",
pages = "949--52",
journal = "ACS Synthetic Biology",
issn = "2161-5063",
publisher = "American Chemical Society",
number = "12",

}

RIS

TY - JOUR

T1 - Natural LacI from E. coli yields faster response and higher level of expression than the LVA-tagged LacI

AU - Andreassen, Patrick Rosendahl

AU - Fredberg, Sofie

AU - Horan, Mattias

AU - Knudsen, Maria Højberg

AU - Jacobsen, Kirstine

AU - Kjær, Andreas

AU - Krogh, Thøger Jensen

AU - Kronborg, Tina

AU - Mattsson, Nicky Cordua

AU - Schmidt, Sissel Ida

AU - Wille, Heidi

AU - Andersen, Ann

PY - 2014/12/19

Y1 - 2014/12/19

N2 - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

AB - The lac promoter is one of the most commonly used promoters for expression control of recombinant genes in E. coli. In the absence of galactosides, the lac promoter is repressed by its repressor protein LacI. Since the lac promoter is regulated by a repressor, overexpression of LacI is necessary for regulation when the promoter is introduced on a high-copy plasmid. For that purpose, a modified variant of LacI, a LVA-tagged LacI, was submitted to the Registry of Standard Biological Parts and has been used for more than 500 constructs since then. We have found, however, that natural LacI is superior to the LVA-tagged LacI as controller of expression.

U2 - 10.1021/sb500031h

DO - 10.1021/sb500031h

M3 - Journal article

C2 - 25524095

VL - 3

SP - 949

EP - 952

JO - ACS Synthetic Biology

JF - ACS Synthetic Biology

SN - 2161-5063

IS - 12

ER -

ID: 139856714