Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

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Standard

Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids. / Poulsen, L K; Pedersen, M F; Malling, H J; Søndergaard, I; Weeke, B.

I: Allergy, Bind 44, Nr. 3, 04.1989, s. 173-80.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Poulsen, LK, Pedersen, MF, Malling, HJ, Søndergaard, I & Weeke, B 1989, 'Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids', Allergy, bind 44, nr. 3, s. 173-80.

APA

Poulsen, L. K., Pedersen, M. F., Malling, H. J., Søndergaard, I., & Weeke, B. (1989). Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids. Allergy, 44(3), 173-80.

Vancouver

Poulsen LK, Pedersen MF, Malling HJ, Søndergaard I, Weeke B. Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids. Allergy. 1989 apr.;44(3):173-80.

Author

Poulsen, L K ; Pedersen, M F ; Malling, H J ; Søndergaard, I ; Weeke, B. / Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids. I: Allergy. 1989 ; Bind 44, Nr. 3. s. 173-80.

Bibtex

@article{f7253121c463413f803e75af032e0f0d,
title = "Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids",
abstract = "For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ({"}Maxisorp RAST{"}). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.",
keywords = "Allergens, Antigens, Fungal, Cladosporium, Epitopes, Humans, Immunoglobulin E, Radioallergosorbent Test, Journal Article, Research Support, Non-U.S. Gov't",
author = "Poulsen, {L K} and Pedersen, {M F} and Malling, {H J} and I S{\o}ndergaard and B Weeke",
year = "1989",
month = apr,
language = "English",
volume = "44",
pages = "173--80",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley Online",
number = "3",

}

RIS

TY - JOUR

T1 - Maxisorp RAST. A sensitive method for detection of antigen-specific human IgE in culture fluids

AU - Poulsen, L K

AU - Pedersen, M F

AU - Malling, H J

AU - Søndergaard, I

AU - Weeke, B

PY - 1989/4

Y1 - 1989/4

N2 - For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

AB - For determination of allergen-specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed ("Maxisorp RAST"). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations (r = 0.67-0.88) with established RAST procedures were found. Based on immunosorbent-purified allergen-specific IgE the estimated sensitivity was within the order of 150-300 pg allergen-specific IgE per ml. The within-assay variation was 4-9% and the inter-assay-variation 17-29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.

KW - Allergens

KW - Antigens, Fungal

KW - Cladosporium

KW - Epitopes

KW - Humans

KW - Immunoglobulin E

KW - Radioallergosorbent Test

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 2469345

VL - 44

SP - 173

EP - 180

JO - Allergy: European Journal of Allergy and Clinical Immunology

JF - Allergy: European Journal of Allergy and Clinical Immunology

SN - 0105-4538

IS - 3

ER -

ID: 169715030