Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia
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Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia. / Christensen, Henrik; Kuhnert, Peter; Foster, Geoffrey; Bisgaard, Magne.
I: International Journal of Systematic and Evolutionary Microbiology, Bind 74, Nr. 5, 006370, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Mannheimia indoligenes sp. nov., proposed for clade V organisms of Mannheimia
AU - Christensen, Henrik
AU - Kuhnert, Peter
AU - Foster, Geoffrey
AU - Bisgaard, Magne
N1 - Publisher Copyright: © 2024 The Authors.
PY - 2024
Y1 - 2024
N2 - A set of 25 strains belonging to clade V of Mannheimia mainly isolated from cattle was investigated and is proposed to represent Mannheimia indoligenes sp. nov. The species can be separated from the other validly published species of the genus by pheno-and genotype. Only indole separates M. indoligenes and Mannheimia varigena while two to seven characters separate M. indoligenes from other species of Mannheimia. Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98–100% similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA–DNA hybridization showed that the similarities between the suggested type strain M14.4T and the other strains of M. indoligenes were 62.9% or higher. The average nucleotide identity was 95.5% or higher between M14.4T and the other strains of the species. The rpoB gene sequence similarity was 95–100% within M. indoligenes. MALDI-TOF allowed a clear separation from other Mannheimia species further supporting classification as a novel species and making it the diagnostic identification tool of choice for M. indoligenes. The type strain is M14.4T (=CCUG 77347T=DSM 116804T) isolated from a cattle tongue in Scotland.
AB - A set of 25 strains belonging to clade V of Mannheimia mainly isolated from cattle was investigated and is proposed to represent Mannheimia indoligenes sp. nov. The species can be separated from the other validly published species of the genus by pheno-and genotype. Only indole separates M. indoligenes and Mannheimia varigena while two to seven characters separate M. indoligenes from other species of Mannheimia. Thirteen strains belonging to biogroups 6, 7, 8C, 9, 10, 12 and UG5 formed a monophyletic group based on 16S rRNA gene sequence comparisons with 98–100% similarity. Eight of these strains were further included in the whole genome comparison. Digital DNA–DNA hybridization showed that the similarities between the suggested type strain M14.4T and the other strains of M. indoligenes were 62.9% or higher. The average nucleotide identity was 95.5% or higher between M14.4T and the other strains of the species. The rpoB gene sequence similarity was 95–100% within M. indoligenes. MALDI-TOF allowed a clear separation from other Mannheimia species further supporting classification as a novel species and making it the diagnostic identification tool of choice for M. indoligenes. The type strain is M14.4T (=CCUG 77347T=DSM 116804T) isolated from a cattle tongue in Scotland.
U2 - 10.1099/ijsem.0.006370
DO - 10.1099/ijsem.0.006370
M3 - Journal article
C2 - 38739681
VL - 74
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 5
M1 - 006370
ER -
ID: 394540758