Lysosomes: a possible target for psoralen photodamage

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Standard

Lysosomes : a possible target for psoralen photodamage. / Frederiksen, S; Nielsen, Peter E.; Høyer, P E.

I: Journal of photochemistry and photobiology. B, Biology, Bind 3, Nr. 3, 06.1989, s. 437-47.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Frederiksen, S, Nielsen, PE & Høyer, PE 1989, 'Lysosomes: a possible target for psoralen photodamage', Journal of photochemistry and photobiology. B, Biology, bind 3, nr. 3, s. 437-47. https://doi.org/10.1016/1011-1344(89)80048-X

APA

Frederiksen, S., Nielsen, P. E., & Høyer, P. E. (1989). Lysosomes: a possible target for psoralen photodamage. Journal of photochemistry and photobiology. B, Biology, 3(3), 437-47. https://doi.org/10.1016/1011-1344(89)80048-X

Vancouver

Frederiksen S, Nielsen PE, Høyer PE. Lysosomes: a possible target for psoralen photodamage. Journal of photochemistry and photobiology. B, Biology. 1989 jun.;3(3):437-47. https://doi.org/10.1016/1011-1344(89)80048-X

Author

Frederiksen, S ; Nielsen, Peter E. ; Høyer, P E. / Lysosomes : a possible target for psoralen photodamage. I: Journal of photochemistry and photobiology. B, Biology. 1989 ; Bind 3, Nr. 3. s. 437-47.

Bibtex

@article{4336b9c037834210a6620df0209acd52,
title = "Lysosomes: a possible target for psoralen photodamage",
abstract = "Treatment in vitro of Ehrlich ascites tumor cells or human fibroblasts with 8-methoxypsoralen (8-MOP, 2.4 microM) and UVA irradiation results in a 30% and 60% respectively reduction in lysosomal beta-galactosidase activity in situ. Under identical conditions one 8-MOP adduct was formed per 2 X 10(4) bases of DNA, one 8-MOP adduct was formed per approximately 10(4) tRNA molecules and one per approximately 100 ribosomes. It is suggested that the decrease in lysosomal beta-galactosidase activity is a result of leakage through the lysosomal membrane caused by psoralen-UVA damage of the lipids in the membrane, since no effect was found on beta-galactosidase in vitro. These results indicate that the lysosomes may also be a target for cellular photodamage by 8-methoxy-psoralen.",
keywords = "Autoradiography, Chromatography, Thin Layer, DNA/drug effects, Fibroblasts/enzymology, Humans, Lipid Metabolism, Lysosomes/drug effects, Methoxsalen/pharmacology, PUVA Therapy, Proteins/metabolism, RNA/drug effects, Tumor Cells, Cultured/enzymology, beta-Galactosidase/metabolism",
author = "S Frederiksen and Nielsen, {Peter E.} and H{\o}yer, {P E}",
year = "1989",
month = jun,
doi = "10.1016/1011-1344(89)80048-X",
language = "English",
volume = "3",
pages = "437--47",
journal = "Journal of Photochemistry and Photobiology B: Biology",
issn = "1011-1344",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Lysosomes

T2 - a possible target for psoralen photodamage

AU - Frederiksen, S

AU - Nielsen, Peter E.

AU - Høyer, P E

PY - 1989/6

Y1 - 1989/6

N2 - Treatment in vitro of Ehrlich ascites tumor cells or human fibroblasts with 8-methoxypsoralen (8-MOP, 2.4 microM) and UVA irradiation results in a 30% and 60% respectively reduction in lysosomal beta-galactosidase activity in situ. Under identical conditions one 8-MOP adduct was formed per 2 X 10(4) bases of DNA, one 8-MOP adduct was formed per approximately 10(4) tRNA molecules and one per approximately 100 ribosomes. It is suggested that the decrease in lysosomal beta-galactosidase activity is a result of leakage through the lysosomal membrane caused by psoralen-UVA damage of the lipids in the membrane, since no effect was found on beta-galactosidase in vitro. These results indicate that the lysosomes may also be a target for cellular photodamage by 8-methoxy-psoralen.

AB - Treatment in vitro of Ehrlich ascites tumor cells or human fibroblasts with 8-methoxypsoralen (8-MOP, 2.4 microM) and UVA irradiation results in a 30% and 60% respectively reduction in lysosomal beta-galactosidase activity in situ. Under identical conditions one 8-MOP adduct was formed per 2 X 10(4) bases of DNA, one 8-MOP adduct was formed per approximately 10(4) tRNA molecules and one per approximately 100 ribosomes. It is suggested that the decrease in lysosomal beta-galactosidase activity is a result of leakage through the lysosomal membrane caused by psoralen-UVA damage of the lipids in the membrane, since no effect was found on beta-galactosidase in vitro. These results indicate that the lysosomes may also be a target for cellular photodamage by 8-methoxy-psoralen.

KW - Autoradiography

KW - Chromatography, Thin Layer

KW - DNA/drug effects

KW - Fibroblasts/enzymology

KW - Humans

KW - Lipid Metabolism

KW - Lysosomes/drug effects

KW - Methoxsalen/pharmacology

KW - PUVA Therapy

KW - Proteins/metabolism

KW - RNA/drug effects

KW - Tumor Cells, Cultured/enzymology

KW - beta-Galactosidase/metabolism

U2 - 10.1016/1011-1344(89)80048-X

DO - 10.1016/1011-1344(89)80048-X

M3 - Journal article

C2 - 2475602

VL - 3

SP - 437

EP - 447

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

SN - 1011-1344

IS - 3

ER -

ID: 203632304