KCNE4 is an inhibitory subunit to the KCNQ1 channel.
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KCNE4 is an inhibitory subunit to the KCNQ1 channel. / Grunnet, Morten; Jespersen, Thomas; Rasmussen, Hanne Borger; Ljungstrøm, Trine; Jorgensen, Nanna K; Olesen, Søren-Peter; Klaerke, Dan A.
I: Journal of Physiology, Bind 542, Nr. Pt 1, 2002, s. 119-30.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - KCNE4 is an inhibitory subunit to the KCNQ1 channel.
AU - Grunnet, Morten
AU - Jespersen, Thomas
AU - Rasmussen, Hanne Borger
AU - Ljungstrøm, Trine
AU - Jorgensen, Nanna K
AU - Olesen, Søren-Peter
AU - Klaerke, Dan A
N1 - Keywords: Animals; Biotin; CHO Cells; Carrier Proteins; Cation Transport Proteins; Cricetinae; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Electrophysiology; Ether-A-Go-Go Potassium Channels; Female; Immunoblotting; KCNQ Potassium Channels; KCNQ1 Potassium Channel; Membrane Potentials; Membrane Proteins; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Oocytes; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Voltage-Gated; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Sepharose; Streptavidin; Tissue Distribution; Trans-Activators; Xenopus
PY - 2002
Y1 - 2002
N2 - KCNE4 is a membrane protein belonging to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. However, no functional role of KCNE4 has been suggested so far. In the present paper we demonstrate that KCNE4 is an inhibitory subunit to KCNQ1 channels. Co-expression of KCNQ1 and KCNE4 in Xenopus oocytes completely inhibited the KCNQ1 current. This was reproduced in mammalian CHO-K1 cells. Experiments with delayed expression of mRNA coding for KCNE4 in KCNQ1-expressing oocytes suggested that KCNE4 exerts its effect on KCNQ1 channels already expressed in the plasma membrane. This notion was supported by immunocytochemical studies and Western blotting, showing no significant difference in plasma membrane expression of KCNQ1 channels in the presence or absence of KCNE4. The impact of KCNE4 on KCNQ1 was specific since no effect of KCNE4 could be detected if co-expressed with KCNQ2-5 channels or hERG1 channels. RT-PCR studies revealed high KCNE4 expression in embryos and adult uterus, where significant expression of KCNQ1 channels has also been demonstrated.
AB - KCNE4 is a membrane protein belonging to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. However, no functional role of KCNE4 has been suggested so far. In the present paper we demonstrate that KCNE4 is an inhibitory subunit to KCNQ1 channels. Co-expression of KCNQ1 and KCNE4 in Xenopus oocytes completely inhibited the KCNQ1 current. This was reproduced in mammalian CHO-K1 cells. Experiments with delayed expression of mRNA coding for KCNE4 in KCNQ1-expressing oocytes suggested that KCNE4 exerts its effect on KCNQ1 channels already expressed in the plasma membrane. This notion was supported by immunocytochemical studies and Western blotting, showing no significant difference in plasma membrane expression of KCNQ1 channels in the presence or absence of KCNE4. The impact of KCNE4 on KCNQ1 was specific since no effect of KCNE4 could be detected if co-expressed with KCNQ2-5 channels or hERG1 channels. RT-PCR studies revealed high KCNE4 expression in embryos and adult uterus, where significant expression of KCNQ1 channels has also been demonstrated.
M3 - Journal article
C2 - 12096056
VL - 542
SP - 119
EP - 130
JO - The Journal of Physiology
JF - The Journal of Physiology
SN - 0022-3751
IS - Pt 1
ER -
ID: 8419082