Isolation of microRNA targets using biotinylated synthetic microRNAs.

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Standard

Isolation of microRNA targets using biotinylated synthetic microRNAs. / Ørom, Ulf Andersson; Lund, Anders H.

I: Methods, Bind 43, Nr. 2, 2007, s. 162-5.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Ørom, UA & Lund, AH 2007, 'Isolation of microRNA targets using biotinylated synthetic microRNAs.', Methods, bind 43, nr. 2, s. 162-5. https://doi.org/10.1016/j.ymeth.2007.04.007

APA

Ørom, U. A., & Lund, A. H. (2007). Isolation of microRNA targets using biotinylated synthetic microRNAs. Methods, 43(2), 162-5. https://doi.org/10.1016/j.ymeth.2007.04.007

Vancouver

Ørom UA, Lund AH. Isolation of microRNA targets using biotinylated synthetic microRNAs. Methods. 2007;43(2):162-5. https://doi.org/10.1016/j.ymeth.2007.04.007

Author

Ørom, Ulf Andersson ; Lund, Anders H. / Isolation of microRNA targets using biotinylated synthetic microRNAs. I: Methods. 2007 ; Bind 43, Nr. 2. s. 162-5.

Bibtex

@article{b7fcce50d8de11dcbee902004c4f4f50,
title = "Isolation of microRNA targets using biotinylated synthetic microRNAs.",
abstract = "MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct",
author = "{\O}rom, {Ulf Andersson} and Lund, {Anders H}",
note = "Keywords: Animals; Base Sequence; Biotin; Cell Line; Chromatography, Affinity; Drosophila; Drosophila Proteins; Genes, Insect; Humans; MicroRNAs; Neuropeptides; Transfection",
year = "2007",
doi = "10.1016/j.ymeth.2007.04.007",
language = "English",
volume = "43",
pages = "162--5",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation of microRNA targets using biotinylated synthetic microRNAs.

AU - Ørom, Ulf Andersson

AU - Lund, Anders H

N1 - Keywords: Animals; Base Sequence; Biotin; Cell Line; Chromatography, Affinity; Drosophila; Drosophila Proteins; Genes, Insect; Humans; MicroRNAs; Neuropeptides; Transfection

PY - 2007

Y1 - 2007

N2 - MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct

AB - MicroRNAs are small regulatory RNAs found in multicellular organisms where they post-transcriptionally regulate gene expression. In animals, microRNAs bind mRNAs via incomplete base pairings making the identification of microRNA targets inherently difficult. Here, we present a detailed method for experimental identification of microRNA targets based on affinity purification of tagged microRNAs associated with their targets. Udgivelsesdato: 2007-Oct

U2 - 10.1016/j.ymeth.2007.04.007

DO - 10.1016/j.ymeth.2007.04.007

M3 - Journal article

C2 - 17889804

VL - 43

SP - 162

EP - 165

JO - Methods

JF - Methods

SN - 1046-2023

IS - 2

ER -

ID: 2690279