Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm

Publikation: Working paper › Preprint › Forskning

Standard

Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm. / Lindhøj, Mette Bjerg; Cooper, Susan Rudd; Anker, Andy Sode; Bonnin, Anne; Kristensen, Mie; Qvortrup, Klaus; Almstrup, Kristian; Jensen, Kirsten Marie Ørnsbjerg; Dyrby, Tim B; Sporring, Jon.

arxiv.org, 2023.

Publikation: Working paper › Preprint › Forskning

Harvard

Lindhøj, MB, Cooper, SR, Anker, AS, Bonnin, A, Kristensen, M, Qvortrup, K, Almstrup, K, Jensen, KMØ, Dyrby, TB & Sporring, J 2023 'Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm' arxiv.org. https://doi.org/10.48550/arXiv.2309.03908

APA

Lindhøj, M. B., Cooper, S. R., Anker, A. S., Bonnin, A., Kristensen, M., Qvortrup, K., Almstrup, K., Jensen, K. M. Ø., Dyrby, T. B., & Sporring, J. (2023). Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm. arxiv.org. https://doi.org/10.48550/arXiv.2309.03908

Vancouver

Lindhøj MB, Cooper SR, Anker AS, Bonnin A, Kristensen M, Qvortrup K o.a. Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm. arxiv.org. 2023. https://doi.org/10.48550/arXiv.2309.03908

Author

Lindhøj, Mette Bjerg ; Cooper, Susan Rudd ; Anker, Andy Sode ; Bonnin, Anne ; Kristensen, Mie ; Qvortrup, Klaus ; Almstrup, Kristian ; Jensen, Kirsten Marie Ørnsbjerg ; Dyrby, Tim B ; Sporring, Jon. / Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm. arxiv.org, 2023.

Bibtex

@techreport{139a8bd1ad354695aee7185574bcf75d,
title = "Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm",
abstract = "Fast phase-contrast imaging offered by modern synchrotron facilities opens the possibility of imaging dynamic processes of biological material such as cells. Cells are mainly composed of carbon and hydrogen, which have low X-ray attenuation, making cell studies with X-ray tomography challenging. At specific low energies, cells provide contrast, but cryo-conditions are required to protect the sample from radiation damage. Thus, non-toxic labelling methods are needed to prepare living cells for X-ray tomography at higher energies. We propose using iron oxide nanoparticles due to their proven compatibility in other biomedical applications. We show how to synthesize and attach iron oxide nanoparticles and demonstrate that cell-penetrating peptides facilitate iron oxide nanoparticle uptake into sperm cells. We show results from the TOMCAT Nanoscope (Swiss Light Source), showing that iron oxide nanoparticles allow the heads and midpiece of fixed sperm samples to be reconstructed from X-ray projections taken at 10 keV. ",
author = "Lindh{\o}j, {Mette Bjerg} and Cooper, {Susan Rudd} and Anker, {Andy Sode} and Anne Bonnin and Mie Kristensen and Klaus Qvortrup and Kristian Almstrup and Jensen, {Kirsten Marie {\O}rnsbjerg} and Dyrby, {Tim B} and Jon Sporring",
year = "2023",
doi = "10.48550/arXiv.2309.03908",
language = "English",
publisher = "arxiv.org",
type = "WorkingPaper",
institution = "arxiv.org",

}

RIS

TY - UNPB

T1 - Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm

AU - Lindhøj, Mette Bjerg

AU - Cooper, Susan Rudd

AU - Anker, Andy Sode

AU - Bonnin, Anne

AU - Kristensen, Mie

AU - Qvortrup, Klaus

AU - Almstrup, Kristian

AU - Jensen, Kirsten Marie Ørnsbjerg

AU - Dyrby, Tim B

AU - Sporring, Jon

PY - 2023

Y1 - 2023

N2 - Fast phase-contrast imaging offered by modern synchrotron facilities opens the possibility of imaging dynamic processes of biological material such as cells. Cells are mainly composed of carbon and hydrogen, which have low X-ray attenuation, making cell studies with X-ray tomography challenging. At specific low energies, cells provide contrast, but cryo-conditions are required to protect the sample from radiation damage. Thus, non-toxic labelling methods are needed to prepare living cells for X-ray tomography at higher energies. We propose using iron oxide nanoparticles due to their proven compatibility in other biomedical applications. We show how to synthesize and attach iron oxide nanoparticles and demonstrate that cell-penetrating peptides facilitate iron oxide nanoparticle uptake into sperm cells. We show results from the TOMCAT Nanoscope (Swiss Light Source), showing that iron oxide nanoparticles allow the heads and midpiece of fixed sperm samples to be reconstructed from X-ray projections taken at 10 keV.

AB - Fast phase-contrast imaging offered by modern synchrotron facilities opens the possibility of imaging dynamic processes of biological material such as cells. Cells are mainly composed of carbon and hydrogen, which have low X-ray attenuation, making cell studies with X-ray tomography challenging. At specific low energies, cells provide contrast, but cryo-conditions are required to protect the sample from radiation damage. Thus, non-toxic labelling methods are needed to prepare living cells for X-ray tomography at higher energies. We propose using iron oxide nanoparticles due to their proven compatibility in other biomedical applications. We show how to synthesize and attach iron oxide nanoparticles and demonstrate that cell-penetrating peptides facilitate iron oxide nanoparticle uptake into sperm cells. We show results from the TOMCAT Nanoscope (Swiss Light Source), showing that iron oxide nanoparticles allow the heads and midpiece of fixed sperm samples to be reconstructed from X-ray projections taken at 10 keV.

U2 - 10.48550/arXiv.2309.03908

DO - 10.48550/arXiv.2309.03908

M3 - Preprint

BT - Iron Oxide Nanoparticles as a Contrast Agent for Synchrotron Imaging of Sperm

PB - arxiv.org

ER -

ID: 393060206