Increased periodontal bone loss in temporarily B lymphocyte-deficient rats
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Increased periodontal bone loss in temporarily B lymphocyte-deficient rats. / Klausen, B; Hougen, H P; Fiehn, N E.
I: Journal of Periodontal Research, Bind 24, Nr. 6, 11.1989, s. 384-90.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Increased periodontal bone loss in temporarily B lymphocyte-deficient rats
AU - Klausen, B
AU - Hougen, H P
AU - Fiehn, N E
PY - 1989/11
Y1 - 1989/11
N2 - In order to study the role of T lymphocytes and B lymphocytes in the development of marginal periodontitis, experiments were performed on specific-pathogen-free (SPF) rats with various immunologic profiles. The study comprised nude (congenitally T lymphocyte-deficient), thymus-grafted nude (T-lymphocyte reconstituted), anti-mu treated (temporarily B lymphocyte-deficient), and normal rats. When they were 5 to 9 weeks old, some of the rats were inoculated with Actinomyces viscosus. Bacteroides gingivalis and a strain of oral spirochetes; 90 days later they were killed. A. viscosus and B. gingivalis colonized readily, but the spirochete strain could not be established permanently in the oral cavity of the rats, 95% of the inoculated rats raised serum IgG or IgM antibody against one or more of the microorganisms; nude rats tended to raise less antibody than the three other experimental groups. Inoculated rats had significantly less periodontal bone support than controls. Anti-mu treated inoculated rats had significantly less periodontal bone support than nude and normal rats, whereas no difference was found between normal, nude, and thymus-grafted rats. It is concluded that permanent T-lymphocyte deficiency did not interfere with the development of periodontal disease in this model, whereas a temporary and moderate reduction in B-lymphocyte numbers seemed to predispose for aggravation of periodontal bone loss.
AB - In order to study the role of T lymphocytes and B lymphocytes in the development of marginal periodontitis, experiments were performed on specific-pathogen-free (SPF) rats with various immunologic profiles. The study comprised nude (congenitally T lymphocyte-deficient), thymus-grafted nude (T-lymphocyte reconstituted), anti-mu treated (temporarily B lymphocyte-deficient), and normal rats. When they were 5 to 9 weeks old, some of the rats were inoculated with Actinomyces viscosus. Bacteroides gingivalis and a strain of oral spirochetes; 90 days later they were killed. A. viscosus and B. gingivalis colonized readily, but the spirochete strain could not be established permanently in the oral cavity of the rats, 95% of the inoculated rats raised serum IgG or IgM antibody against one or more of the microorganisms; nude rats tended to raise less antibody than the three other experimental groups. Inoculated rats had significantly less periodontal bone support than controls. Anti-mu treated inoculated rats had significantly less periodontal bone support than nude and normal rats, whereas no difference was found between normal, nude, and thymus-grafted rats. It is concluded that permanent T-lymphocyte deficiency did not interfere with the development of periodontal disease in this model, whereas a temporary and moderate reduction in B-lymphocyte numbers seemed to predispose for aggravation of periodontal bone loss.
KW - Actinomyces
KW - Animals
KW - B-Lymphocytes
KW - Bacteroides
KW - Bone Resorption
KW - Female
KW - Immunoglobulin G
KW - Immunoglobulin M
KW - Leukocyte Count
KW - Lymphopenia
KW - Male
KW - Periodontitis
KW - Rats
KW - Rats, Inbred Lew
KW - Rats, Inbred Strains
KW - Rats, Nude
KW - Specific Pathogen-Free Organisms
KW - Spirochaetales
KW - T-Lymphocytes
KW - Thymus Gland
M3 - Journal article
C2 - 2531793
VL - 24
SP - 384
EP - 390
JO - Journal of Periodontal Research
JF - Journal of Periodontal Research
SN - 0022-3484
IS - 6
ER -
ID: 44514556