Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise: use of FLT and PET/CT
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Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise : use of FLT and PET/CT. / Skovgaard, Dorthe; Bayer, Monika L; Mackey, Abigail; Madsen, Jacob; Kjaer, Michael; Kjaer, Andreas.
I: Molecular Imaging and Biology, Bind 12, Nr. 6, 2010, s. 626-634.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise
T2 - use of FLT and PET/CT
AU - Skovgaard, Dorthe
AU - Bayer, Monika L
AU - Mackey, Abigail
AU - Madsen, Jacob
AU - Kjaer, Michael
AU - Kjaer, Andreas
PY - 2010
Y1 - 2010
N2 - PURPOSE: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3'-[F-18]fluoro-3'deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67.PROCEDURES: Wistar rats (n = 13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n = 10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67.RESULTS: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p < 0.001) and in Achilles tendon (21%, p < 0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon.CONCLUSION: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.
AB - PURPOSE: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3'-[F-18]fluoro-3'deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67.PROCEDURES: Wistar rats (n = 13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n = 10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67.RESULTS: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p < 0.001) and in Achilles tendon (21%, p < 0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon.CONCLUSION: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.
KW - Animals
KW - Cell Proliferation
KW - Dideoxynucleosides
KW - Exercise Test
KW - Hyperplasia
KW - Ki-67 Antigen
KW - Male
KW - Muscle, Skeletal
KW - Physical Conditioning, Animal
KW - Positron-Emission Tomography
KW - Rats
KW - Rats, Wistar
KW - Running
KW - Tendons
KW - Tomography, Emission-Computed
KW - Up-Regulation
U2 - 10.1007/s11307-010-0316-y
DO - 10.1007/s11307-010-0316-y
M3 - Journal article
C2 - 20379786
VL - 12
SP - 626
EP - 634
JO - Molecular Imaging and Biology
JF - Molecular Imaging and Biology
SN - 1536-1632
IS - 6
ER -
ID: 34113802