In-chip fabrication of free-form 3D constructs for directed cell migration analysis
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In-chip fabrication of free-form 3D constructs for directed cell migration analysis. / Olsen, Mark Holm; Hjortø, Gertrud Malene; Hansen, Morten; Met, Özcan; Svane, Inge Marie; Larsen, Niels B.
I: Lab On a Chip, Bind 13, Nr. 24, 21.12.2013, s. 4800-4809.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - In-chip fabrication of free-form 3D constructs for directed cell migration analysis
AU - Olsen, Mark Holm
AU - Hjortø, Gertrud Malene
AU - Hansen, Morten
AU - Met, Özcan
AU - Svane, Inge Marie
AU - Larsen, Niels B.
PY - 2013/12/21
Y1 - 2013/12/21
N2 - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.
AB - Free-form constructs with three-dimensional (3D) microporosity were fabricated by two-photon polymerization inside the closed microchannel of an injection-molded, commercially available polymer chip for analysis of directed cell migration. Acrylate constructs were produced as woodpile topologies with a range of pore sizes from 5 × 5 μm to 15 × 15 μm and prefilled with fibrillar collagen. Dendritic cells seeded into the polymer chip in a concentration gradient of the chemoattractant CCL21 efficiently negotiated the microporous maze structure for pore sizes of 8 × 8 μm or larger. The cells migrating through smaller pore sizes made significantly more turns than those through larger pores. The introduction of additional defined barriers in the microporous structure resulted in dendritic cells making more turns while still being able to follow the chemoattractant concentration gradient.
UR - http://www.scopus.com/inward/record.url?scp=84887516677&partnerID=8YFLogxK
U2 - 10.1039/c3lc50930c
DO - 10.1039/c3lc50930c
M3 - Journal article
C2 - 24153393
AN - SCOPUS:84887516677
VL - 13
SP - 4800
EP - 4809
JO - Lab on a Chip
JF - Lab on a Chip
SN - 1473-0197
IS - 24
ER -
ID: 176376008