Improvements in the transformation of Arabidopsis thaliana C24 leaf-discs by Agrobacterium tumefaciens
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We report here an efficient Arabidopsis leafdisc transformation protocol yielding an average transformation frequency of 1.6 transgenic shoots per leaf explant 4 weeks after the bacterial infection period. Subsequent cultivation in vitro is such that a high percentage (85-90%) of the primary transformants produces seeds with an average seed yield of 100-300 seeds per plant. This improved transformation protocol yields mainly (70%) transformants segregating for a single T-DNA locus of which 68% actually contain one T-DNA insert. The objective is to generate a pool of independent transformants harboring an activator T-DNA construct in a gene tagging approach to isolate genes involved in morphogenesis and auxin signal transduction.
Originalsprog | Engelsk |
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Tidsskrift | Plant Cell Reports |
Vol/bind | 15 |
Udgave nummer | 8 |
Sider (fra-til) | 572-7 |
Antal sider | 6 |
ISSN | 0721-7714 |
DOI | |
Status | Udgivet - apr. 1996 |
ID: 106392006