Immunological methods for the detection and determination of connective tissue proteoglycans.

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Standard

Immunological methods for the detection and determination of connective tissue proteoglycans. / Caterson, B; Baker, J R; Christner, J E; Couchman, J R.

I: Journal of Investigative Dermatology, Bind 79 Suppl 1, 1982, s. 45s-50s.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Caterson, B, Baker, JR, Christner, JE & Couchman, JR 1982, 'Immunological methods for the detection and determination of connective tissue proteoglycans.', Journal of Investigative Dermatology, bind 79 Suppl 1, s. 45s-50s.

APA

Caterson, B., Baker, J. R., Christner, J. E., & Couchman, J. R. (1982). Immunological methods for the detection and determination of connective tissue proteoglycans. Journal of Investigative Dermatology, 79 Suppl 1, 45s-50s.

Vancouver

Caterson B, Baker JR, Christner JE, Couchman JR. Immunological methods for the detection and determination of connective tissue proteoglycans. Journal of Investigative Dermatology. 1982;79 Suppl 1:45s-50s.

Author

Caterson, B ; Baker, J R ; Christner, J E ; Couchman, J R. / Immunological methods for the detection and determination of connective tissue proteoglycans. I: Journal of Investigative Dermatology. 1982 ; Bind 79 Suppl 1. s. 45s-50s.

Bibtex

@article{a68b3800598f11dd8d9f000ea68e967b,
title = "Immunological methods for the detection and determination of connective tissue proteoglycans.",
abstract = "In this paper we report the use of immunological methods for specifically detecting and determining proteoglycan in cartilage and other connective tissues. Antibodies (polyclonal and monoclonal) have been raised against specific components of cartilage proteoglycan aggregates (i.e., proteoglycan monomer and link protein). Radioimmunoassay procedures and immunohistochemical procedures have been developed and used to demonstrate the occurrence of cartilage-like proteoglycan and link protein in bovine aorta. Similarly, immunofluorescent studies have been used to analyze proteoglycan distribution in skin. Using antibodies specific for chondroitin-4-sulfated proteoglycan, their presence was demonstrated in dermal connective tissue and connective tissue surrounding nerve and muscle sheaths. However, chondroitin-4-sulfated proteoglycan was completely absent in the epidermis of skin and areas surrounding invaginating hair follicles. These immunological procedures are currently being used to complement conventional biochemical analyses of proteoglycans found in different connective tissue matrices.",
author = "B Caterson and Baker, {J R} and Christner, {J E} and Couchman, {J R}",
note = "Keywords: Animals; Antibodies, Monoclonal; Cartilage; Cattle; Chondroitin Sulfates; Connective Tissue; Extracellular Matrix Proteins; Fluorescent Antibody Technique; Proteins; Proteoglycans; Radioimmunoassay; Rats",
year = "1982",
language = "English",
volume = "79 Suppl 1",
pages = "45s--50s",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Immunological methods for the detection and determination of connective tissue proteoglycans.

AU - Caterson, B

AU - Baker, J R

AU - Christner, J E

AU - Couchman, J R

N1 - Keywords: Animals; Antibodies, Monoclonal; Cartilage; Cattle; Chondroitin Sulfates; Connective Tissue; Extracellular Matrix Proteins; Fluorescent Antibody Technique; Proteins; Proteoglycans; Radioimmunoassay; Rats

PY - 1982

Y1 - 1982

N2 - In this paper we report the use of immunological methods for specifically detecting and determining proteoglycan in cartilage and other connective tissues. Antibodies (polyclonal and monoclonal) have been raised against specific components of cartilage proteoglycan aggregates (i.e., proteoglycan monomer and link protein). Radioimmunoassay procedures and immunohistochemical procedures have been developed and used to demonstrate the occurrence of cartilage-like proteoglycan and link protein in bovine aorta. Similarly, immunofluorescent studies have been used to analyze proteoglycan distribution in skin. Using antibodies specific for chondroitin-4-sulfated proteoglycan, their presence was demonstrated in dermal connective tissue and connective tissue surrounding nerve and muscle sheaths. However, chondroitin-4-sulfated proteoglycan was completely absent in the epidermis of skin and areas surrounding invaginating hair follicles. These immunological procedures are currently being used to complement conventional biochemical analyses of proteoglycans found in different connective tissue matrices.

AB - In this paper we report the use of immunological methods for specifically detecting and determining proteoglycan in cartilage and other connective tissues. Antibodies (polyclonal and monoclonal) have been raised against specific components of cartilage proteoglycan aggregates (i.e., proteoglycan monomer and link protein). Radioimmunoassay procedures and immunohistochemical procedures have been developed and used to demonstrate the occurrence of cartilage-like proteoglycan and link protein in bovine aorta. Similarly, immunofluorescent studies have been used to analyze proteoglycan distribution in skin. Using antibodies specific for chondroitin-4-sulfated proteoglycan, their presence was demonstrated in dermal connective tissue and connective tissue surrounding nerve and muscle sheaths. However, chondroitin-4-sulfated proteoglycan was completely absent in the epidermis of skin and areas surrounding invaginating hair follicles. These immunological procedures are currently being used to complement conventional biochemical analyses of proteoglycans found in different connective tissue matrices.

M3 - Journal article

C2 - 6806399

VL - 79 Suppl 1

SP - 45s-50s

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

ER -

ID: 5167889