Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection
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Background: Cervical cancer is an abnormal growth of cervical tissue epithelial cells due to persistent human papilloma virus (HPV) infection. Cynomolgus monkeys (Macaca fascicularis) can be naturally and spontaneously infected with M. fascicularis Papillomavirus Type 3 (MfPV3), a virus that is phylogenetically closely related to human oncogenic HPV (HPV-16 and HPV-34), and therefore a potentially beneficial for modeling HPV disease. This study aims to evaluate the expression of the integrin alpha 6 (ITGα6) receptor in cynomolgus monkeys spontaneously infected with MfPV3, which this receptor also found in human infected with HPV. Methods: The study was done on archived Formalin-fixed Paraffin-Embedded (FFPE) samples of uterine and cervix tissue of cynomolgus monkeys. Immunohistochemistry was also performed to quantify the expression levels of ITGα6. Results: The results showed 80% of the samples positive Cervical Intraepithelial Neoplasia (CIN) and increased expression of ITGα6 significantly in Positive-MfPV3 group than negative-MfPV3 group. Conclusions: This indicated the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection type.
Originalsprog | Engelsk |
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Artikelnummer | e12690 |
Tidsskrift | Journal of Medical Primatology |
Vol/bind | 53 |
Udgave nummer | 1 |
Antal sider | 6 |
ISSN | 0047-2565 |
DOI | |
Status | Udgivet - 2024 |
Bibliografisk note
Funding Information:
We thank Dr Uus Saepuloh, Dr Sela Septima and Isti Kartika Sari, MSi for support regarding molecular technique; the staffs of the Pathology Laboratory and Research Animal Facility at Lodaya, of the Primate Research Center at Bogor Agricultural University for their technical contributions and drh Suryo Saputro assistance in manuscript preparation. This study was financially supported by the National Research and Innovation Agency on RIIM Chapter 3 (Contract Id. 4/IV/KS/05/2023 and No. 13955/IT3/PT.01.03/P/B/2023) May 8, 2023.
Publisher Copyright:
© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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