Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection
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Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection. / Setyawaty, Dyah; Mariya, Silmi; Prabandari, Silvia A.; Boilessen, Ditte Rahbaek; Holst, Peter Johannes; Darusman, Huda Shalahudin.
I: Journal of Medical Primatology, Bind 53, Nr. 1, e12690, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Immunohistochemical characterization of integrin alfa 6 in uterus and cervix of the cynomolgus monkeys (Macaca fascicularis) with MfPV3 infection
AU - Setyawaty, Dyah
AU - Mariya, Silmi
AU - Prabandari, Silvia A.
AU - Boilessen, Ditte Rahbaek
AU - Holst, Peter Johannes
AU - Darusman, Huda Shalahudin
N1 - Publisher Copyright: © 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2024
Y1 - 2024
N2 - Background: Cervical cancer is an abnormal growth of cervical tissue epithelial cells due to persistent human papilloma virus (HPV) infection. Cynomolgus monkeys (Macaca fascicularis) can be naturally and spontaneously infected with M. fascicularis Papillomavirus Type 3 (MfPV3), a virus that is phylogenetically closely related to human oncogenic HPV (HPV-16 and HPV-34), and therefore a potentially beneficial for modeling HPV disease. This study aims to evaluate the expression of the integrin alpha 6 (ITGα6) receptor in cynomolgus monkeys spontaneously infected with MfPV3, which this receptor also found in human infected with HPV. Methods: The study was done on archived Formalin-fixed Paraffin-Embedded (FFPE) samples of uterine and cervix tissue of cynomolgus monkeys. Immunohistochemistry was also performed to quantify the expression levels of ITGα6. Results: The results showed 80% of the samples positive Cervical Intraepithelial Neoplasia (CIN) and increased expression of ITGα6 significantly in Positive-MfPV3 group than negative-MfPV3 group. Conclusions: This indicated the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection type.
AB - Background: Cervical cancer is an abnormal growth of cervical tissue epithelial cells due to persistent human papilloma virus (HPV) infection. Cynomolgus monkeys (Macaca fascicularis) can be naturally and spontaneously infected with M. fascicularis Papillomavirus Type 3 (MfPV3), a virus that is phylogenetically closely related to human oncogenic HPV (HPV-16 and HPV-34), and therefore a potentially beneficial for modeling HPV disease. This study aims to evaluate the expression of the integrin alpha 6 (ITGα6) receptor in cynomolgus monkeys spontaneously infected with MfPV3, which this receptor also found in human infected with HPV. Methods: The study was done on archived Formalin-fixed Paraffin-Embedded (FFPE) samples of uterine and cervix tissue of cynomolgus monkeys. Immunohistochemistry was also performed to quantify the expression levels of ITGα6. Results: The results showed 80% of the samples positive Cervical Intraepithelial Neoplasia (CIN) and increased expression of ITGα6 significantly in Positive-MfPV3 group than negative-MfPV3 group. Conclusions: This indicated the potential of cynomolgus monkeys as a spontaneous oncogenesis model of PV infection type.
KW - cynomolgus monkeys
KW - human papilloma virus
KW - immunohistochemistry
KW - ITGα6
U2 - 10.1111/jmp.12690
DO - 10.1111/jmp.12690
M3 - Journal article
C2 - 38345331
AN - SCOPUS:85182478571
VL - 53
JO - Journal of Medical Primatology
JF - Journal of Medical Primatology
SN - 0047-2565
IS - 1
M1 - e12690
ER -
ID: 389796096