Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

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Standard

Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine. / Poulsen, L K; Nolte, H; Søndergaard, I; Stahl Skov, P.

I: Agents and Actions, Bind 30, Nr. 3-4, 06.1990, s. 313-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Poulsen, LK, Nolte, H, Søndergaard, I & Stahl Skov, P 1990, 'Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine', Agents and Actions, bind 30, nr. 3-4, s. 313-8.

APA

Poulsen, L. K., Nolte, H., Søndergaard, I., & Stahl Skov, P. (1990). Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine. Agents and Actions, 30(3-4), 313-8.

Vancouver

Poulsen LK, Nolte H, Søndergaard I, Stahl Skov P. Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine. Agents and Actions. 1990 jun.;30(3-4):313-8.

Author

Poulsen, L K ; Nolte, H ; Søndergaard, I ; Stahl Skov, P. / Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine. I: Agents and Actions. 1990 ; Bind 30, Nr. 3-4. s. 313-8.

Bibtex

@article{b8c27b7037754d99849ac3f71acd1f5d,
title = "Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine",
abstract = "For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.",
keywords = "Adsorption, Animals, Antibody Specificity, Cross Reactions, Histamine, Humans, Immunization, Immunoradiometric Assay, Immunosorbents, Iodine Radioisotopes, Rabbits, Serum Albumin, Staphylococcal Protein A, Journal Article, Research Support, Non-U.S. Gov't",
author = "Poulsen, {L K} and H Nolte and I S{\o}ndergaard and {Stahl Skov}, P",
year = "1990",
month = jun,
language = "English",
volume = "30",
pages = "313--8",
journal = "Agents and Actions",
issn = "0065-4299",
publisher = "Birkhauser Verlag Basel",
number = "3-4",

}

RIS

TY - JOUR

T1 - Immunochemical cross-reactivity between albumin and solid-phase adsorbed histamine

AU - Poulsen, L K

AU - Nolte, H

AU - Søndergaard, I

AU - Stahl Skov, P

PY - 1990/6

Y1 - 1990/6

N2 - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.

AB - For production of an antibody against histamine, this was coupled to human serum albumin (HSA) and used for immunization of rabbits. To test the antiserum, an immunoradiometric assay was developed comprising solid-phase bound histamine, antisera and radiolabelled protein A. Titration and inhibition experiments revealed that histamine adsorbed onto a solid-phase could bind the antiserum. However, neither free histamine nor histamine coupled to unrelated carriers could inhibit the binding of antiserum to the solid-phase histamine. Cross-reactivity was demonstrated between HSA and solid-phase bound histamine, as the immunoradiometric assay was inhibited by HSA. This unexpected cross-reactivity was established, as a commercially available antiserum with specificity to HSA without histamine also bound to the solid-phase bound histamine. It is suggested that preparations of antibodies against histamine are tested for this possible cross-reactivity.

KW - Adsorption

KW - Animals

KW - Antibody Specificity

KW - Cross Reactions

KW - Histamine

KW - Humans

KW - Immunization

KW - Immunoradiometric Assay

KW - Immunosorbents

KW - Iodine Radioisotopes

KW - Rabbits

KW - Serum Albumin

KW - Staphylococcal Protein A

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 2386106

VL - 30

SP - 313

EP - 318

JO - Agents and Actions

JF - Agents and Actions

SN - 0065-4299

IS - 3-4

ER -

ID: 169715257