Image-Enabled Cell Sorting Using the BD CellView Technology
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Image-Enabled Cell Sorting Using the BD CellView Technology. / Paulsen, Malte S.
Flow Cytometry Protocols. red. / Teresa S. Hawley; Robert G. Hawley. 5. udg. Humana Press, 2024. s. 145-158 (Methods in Molecular Biology, Bind 2779).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Image-Enabled Cell Sorting Using the BD CellView Technology
AU - Paulsen, Malte S.
N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
PY - 2024
Y1 - 2024
N2 - This chapter is an extension of the original publication by Schraivogel et al. (Science 375:315–320, 2022) which described, for the first time, image-enabled and high-speed cell sorting based on the BD CellView technology. It summarizes the technical aspects of the instrument in an easy-to-digest form and provides example-based guidance toward implementation of the CellView-based image cell sorting technology. As an example, it explains how to use the image-enabled cell sorter to analyze the chemically induced fragmentation of the Golgi apparatus in HeLa cells—an experiment that was alluded to in the original publication but was not included in the manuscript due to space constraints. The chemically induced Golgi fragmentation sort illustrates an elegant example of the utility of image-enabled cell sorting as a significant expansion of the single-cell toolbox. It is such a striking phenotype when analyzed with image cytometry but undetectable when using conventional flow cytometry. Described in a straightforward and concise manner, this experiment serves as a standard system assurance for image-based cell sorters.
AB - This chapter is an extension of the original publication by Schraivogel et al. (Science 375:315–320, 2022) which described, for the first time, image-enabled and high-speed cell sorting based on the BD CellView technology. It summarizes the technical aspects of the instrument in an easy-to-digest form and provides example-based guidance toward implementation of the CellView-based image cell sorting technology. As an example, it explains how to use the image-enabled cell sorter to analyze the chemically induced fragmentation of the Golgi apparatus in HeLa cells—an experiment that was alluded to in the original publication but was not included in the manuscript due to space constraints. The chemically induced Golgi fragmentation sort illustrates an elegant example of the utility of image-enabled cell sorting as a significant expansion of the single-cell toolbox. It is such a striking phenotype when analyzed with image cytometry but undetectable when using conventional flow cytometry. Described in a straightforward and concise manner, this experiment serves as a standard system assurance for image-based cell sorters.
KW - Chemically induced Golgi fragmentation
KW - Flow cytometry
KW - High-speed cell sorting
KW - Image-enabled cell sorting
KW - On the fly image analysis parameters
U2 - 10.1007/978-1-0716-3738-8_8
DO - 10.1007/978-1-0716-3738-8_8
M3 - Book chapter
C2 - 38526786
AN - SCOPUS:85189059554
SN - 978-1-0716-3737-1
T3 - Methods in Molecular Biology
SP - 145
EP - 158
BT - Flow Cytometry Protocols
A2 - Hawley, Teresa S.
A2 - Hawley, Robert G.
PB - Humana Press
ER -
ID: 388585370