Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates

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Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates. / Yee, Sook Wah; Ferrández-Peral, Luis; Alentorn-Moron, Pol; Fontsere, Claudia; Ceylan, Merve; Koleske, Megan L.; Handin, Niklas; Artegoitia, Virginia M.; Lara, Giovanni; Chien, Huan-Chieh; Zhou, Xujia; Dainat, Jacques; Zalevsky, Arthur; Sali, Andrej; Brand, Colin M.; Wolfreys, Finn D.; Yang, Jia; Gestwicki, Jason E.; Capra, John A.; Artursson, Per; Newman, John W.; Marquès-Bonet, Tomàs; Giacomini, Kathleen M.

I: Nature Communications, Bind 15, Nr. 1, 4380, 2024.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Yee, SW, Ferrández-Peral, L, Alentorn-Moron, P, Fontsere, C, Ceylan, M, Koleske, ML, Handin, N, Artegoitia, VM, Lara, G, Chien, H-C, Zhou, X, Dainat, J, Zalevsky, A, Sali, A, Brand, CM, Wolfreys, FD, Yang, J, Gestwicki, JE, Capra, JA, Artursson, P, Newman, JW, Marquès-Bonet, T & Giacomini, KM 2024, 'Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates', Nature Communications, bind 15, nr. 1, 4380. https://doi.org/10.1038/s41467-024-48569-7

APA

Yee, S. W., Ferrández-Peral, L., Alentorn-Moron, P., Fontsere, C., Ceylan, M., Koleske, M. L., Handin, N., Artegoitia, V. M., Lara, G., Chien, H-C., Zhou, X., Dainat, J., Zalevsky, A., Sali, A., Brand, C. M., Wolfreys, F. D., Yang, J., Gestwicki, J. E., Capra, J. A., ... Giacomini, K. M. (2024). Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates. Nature Communications, 15(1), [4380]. https://doi.org/10.1038/s41467-024-48569-7

Vancouver

Yee SW, Ferrández-Peral L, Alentorn-Moron P, Fontsere C, Ceylan M, Koleske ML o.a. Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates. Nature Communications. 2024;15(1). 4380. https://doi.org/10.1038/s41467-024-48569-7

Author

Yee, Sook Wah ; Ferrández-Peral, Luis ; Alentorn-Moron, Pol ; Fontsere, Claudia ; Ceylan, Merve ; Koleske, Megan L. ; Handin, Niklas ; Artegoitia, Virginia M. ; Lara, Giovanni ; Chien, Huan-Chieh ; Zhou, Xujia ; Dainat, Jacques ; Zalevsky, Arthur ; Sali, Andrej ; Brand, Colin M. ; Wolfreys, Finn D. ; Yang, Jia ; Gestwicki, Jason E. ; Capra, John A. ; Artursson, Per ; Newman, John W. ; Marquès-Bonet, Tomàs ; Giacomini, Kathleen M. / Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates. I: Nature Communications. 2024 ; Bind 15, Nr. 1.

Bibtex

@article{2804b3724760477c8be0b86dee827c7f,
title = "Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates",
abstract = "SLC22A10 is an orphan transporter with unknown substrates and function. The goal of this study is to elucidate its substrate specificity and functional characteristics. In contrast to orthologs from great apes, human SLC22A10, tagged with green fluorescent protein, is not expressed on the plasma membrane. Cells expressing great ape SLC22A10 orthologs exhibit significant accumulation of estradiol-17β-glucuronide, unlike those expressing human SLC22A10. Sequence alignments reveal a proline at position 220 in humans, which is a leucine in great apes. Replacing proline with leucine in SLC22A10-P220L restores plasma membrane localization and uptake function. Neanderthal and Denisovan genomes show proline at position 220, akin to modern humans, indicating functional loss during hominin evolution. Human SLC22A10 is a unitary pseudogene due to a fixed missense mutation, P220, while in great apes, its orthologs transport sex steroid conjugates. Characterizing SLC22A10 across species sheds light on its biological role, influencing organism development and steroid homeostasis.",
author = "Yee, {Sook Wah} and Luis Ferr{\'a}ndez-Peral and Pol Alentorn-Moron and Claudia Fontsere and Merve Ceylan and Koleske, {Megan L.} and Niklas Handin and Artegoitia, {Virginia M.} and Giovanni Lara and Huan-Chieh Chien and Xujia Zhou and Jacques Dainat and Arthur Zalevsky and Andrej Sali and Brand, {Colin M.} and Wolfreys, {Finn D.} and Jia Yang and Gestwicki, {Jason E.} and Capra, {John A.} and Per Artursson and Newman, {John W.} and Tom{\`a}s Marqu{\`e}s-Bonet and Giacomini, {Kathleen M.}",
note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",
year = "2024",
doi = "10.1038/s41467-024-48569-7",
language = "English",
volume = "15",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Illuminating the function of the orphan transporter, SLC22A10, in humans and other primates

AU - Yee, Sook Wah

AU - Ferrández-Peral, Luis

AU - Alentorn-Moron, Pol

AU - Fontsere, Claudia

AU - Ceylan, Merve

AU - Koleske, Megan L.

AU - Handin, Niklas

AU - Artegoitia, Virginia M.

AU - Lara, Giovanni

AU - Chien, Huan-Chieh

AU - Zhou, Xujia

AU - Dainat, Jacques

AU - Zalevsky, Arthur

AU - Sali, Andrej

AU - Brand, Colin M.

AU - Wolfreys, Finn D.

AU - Yang, Jia

AU - Gestwicki, Jason E.

AU - Capra, John A.

AU - Artursson, Per

AU - Newman, John W.

AU - Marquès-Bonet, Tomàs

AU - Giacomini, Kathleen M.

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024

Y1 - 2024

N2 - SLC22A10 is an orphan transporter with unknown substrates and function. The goal of this study is to elucidate its substrate specificity and functional characteristics. In contrast to orthologs from great apes, human SLC22A10, tagged with green fluorescent protein, is not expressed on the plasma membrane. Cells expressing great ape SLC22A10 orthologs exhibit significant accumulation of estradiol-17β-glucuronide, unlike those expressing human SLC22A10. Sequence alignments reveal a proline at position 220 in humans, which is a leucine in great apes. Replacing proline with leucine in SLC22A10-P220L restores plasma membrane localization and uptake function. Neanderthal and Denisovan genomes show proline at position 220, akin to modern humans, indicating functional loss during hominin evolution. Human SLC22A10 is a unitary pseudogene due to a fixed missense mutation, P220, while in great apes, its orthologs transport sex steroid conjugates. Characterizing SLC22A10 across species sheds light on its biological role, influencing organism development and steroid homeostasis.

AB - SLC22A10 is an orphan transporter with unknown substrates and function. The goal of this study is to elucidate its substrate specificity and functional characteristics. In contrast to orthologs from great apes, human SLC22A10, tagged with green fluorescent protein, is not expressed on the plasma membrane. Cells expressing great ape SLC22A10 orthologs exhibit significant accumulation of estradiol-17β-glucuronide, unlike those expressing human SLC22A10. Sequence alignments reveal a proline at position 220 in humans, which is a leucine in great apes. Replacing proline with leucine in SLC22A10-P220L restores plasma membrane localization and uptake function. Neanderthal and Denisovan genomes show proline at position 220, akin to modern humans, indicating functional loss during hominin evolution. Human SLC22A10 is a unitary pseudogene due to a fixed missense mutation, P220, while in great apes, its orthologs transport sex steroid conjugates. Characterizing SLC22A10 across species sheds light on its biological role, influencing organism development and steroid homeostasis.

U2 - 10.1038/s41467-024-48569-7

DO - 10.1038/s41467-024-48569-7

M3 - Journal article

C2 - 38782905

AN - SCOPUS:85194126861

VL - 15

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 4380

ER -

ID: 395142564