IgG autoantibodies against interleukin 1 alpha in sera of normal individuals
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IgG autoantibodies against interleukin 1 alpha in sera of normal individuals. / Svenson, M; Poulsen, L K; Fomsgaard, A; Bendtzen, K.
I: Scandinavian Journal of Immunology, Bind 29, Nr. 4, 04.1989, s. 489-92.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - IgG autoantibodies against interleukin 1 alpha in sera of normal individuals
AU - Svenson, M
AU - Poulsen, L K
AU - Fomsgaard, A
AU - Bendtzen, K
PY - 1989/4
Y1 - 1989/4
N2 - A pool of human sera from healthy blood donors was found to interfere competitively with the binding of 125I-labelled human recombinant interleukin 1 alpha (rIL-1 alpha) to the murine T-cell line EL4. The interference was reversible at the cellular level, and direct binding of the ligand to serum factors was therefore investigated. After preincubation of [125I]rIL-1 alpha with pooled serum, the 125I activity eluted in two peaks from a Sephadex G-75 column. The first was located in the void volume. The second eluted together with monomer rIL-1 alpha. An almost complete displacement of the high molecular weight 125I fraction was achieved with an excess of unlabelled rIL-1 alpha but not with rIL-1 beta. The serum factors binding to [125I]rIL-1 alpha were located in the molecular weight range 100,000-200,000, judged by fractionation on a Sephacryl S-400 column, and the factors were bound to immobilized protein A. Furthermore, [125I]rIL-1 alpha preincubated with serum co-precipitated with a specific rabbit anti-human IgG antibody. Screening of 29 sera from normal individuals showed similar effects in three cases. We conclude that approximately 10% of normal human sera contains detectable IgG autoantibodies to IL-1 alpha.
AB - A pool of human sera from healthy blood donors was found to interfere competitively with the binding of 125I-labelled human recombinant interleukin 1 alpha (rIL-1 alpha) to the murine T-cell line EL4. The interference was reversible at the cellular level, and direct binding of the ligand to serum factors was therefore investigated. After preincubation of [125I]rIL-1 alpha with pooled serum, the 125I activity eluted in two peaks from a Sephadex G-75 column. The first was located in the void volume. The second eluted together with monomer rIL-1 alpha. An almost complete displacement of the high molecular weight 125I fraction was achieved with an excess of unlabelled rIL-1 alpha but not with rIL-1 beta. The serum factors binding to [125I]rIL-1 alpha were located in the molecular weight range 100,000-200,000, judged by fractionation on a Sephacryl S-400 column, and the factors were bound to immobilized protein A. Furthermore, [125I]rIL-1 alpha preincubated with serum co-precipitated with a specific rabbit anti-human IgG antibody. Screening of 29 sera from normal individuals showed similar effects in three cases. We conclude that approximately 10% of normal human sera contains detectable IgG autoantibodies to IL-1 alpha.
KW - Adult
KW - Animals
KW - Autoantibodies
KW - Binding Sites, Antibody
KW - Cell Line
KW - Chromatography, Gel
KW - Humans
KW - Immune Sera
KW - Immunoglobulin G
KW - Interleukin-1
KW - Mice
KW - Molecular Weight
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
M3 - Journal article
C2 - 2785711
VL - 29
SP - 489
EP - 492
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 4
ER -
ID: 169715065