Identification of thioredoxin target disulfides in proteins released from barley aleurone layers
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Identification of thioredoxin target disulfides in proteins released from barley aleurone layers. / Hägglund, Per; Bunkenborg, Jakob; Yang, Fen; Harder, Lea Mørch; Finnie, Christine; Svensson, Birte.
I: Journal of Proteomics, Bind 73, Nr. 6, 2010, s. 1133-1136.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Identification of thioredoxin target disulfides in proteins released from barley aleurone layers
AU - Hägglund, Per
AU - Bunkenborg, Jakob
AU - Yang, Fen
AU - Harder, Lea Mørch
AU - Finnie, Christine
AU - Svensson, Birte
PY - 2010
Y1 - 2010
N2 - Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the starchy endosperm. Here we have applied a thiol-specific labeling approach to identify specific disulfide targets of barley thioredoxin in proteins released from barley aleurone layers incubated in buffer containing gibberellic acid.
AB - Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the starchy endosperm. Here we have applied a thiol-specific labeling approach to identify specific disulfide targets of barley thioredoxin in proteins released from barley aleurone layers incubated in buffer containing gibberellic acid.
KW - Aleurone layer
KW - Barley germination
KW - Disulfide
KW - Isotope-coded affinity tag
KW - Redox proteomics
KW - Thioredoxin
U2 - 10.1016/j.jprot.2010.01.007
DO - 10.1016/j.jprot.2010.01.007
M3 - Journal article
C2 - 20114090
AN - SCOPUS:77950626738
VL - 73
SP - 1133
EP - 1136
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 6
ER -
ID: 240160404