Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

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Standard

Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF. / Schwartz, J; Billestrup, Nils; Perrin, M; Rivier, J; Vale, W.

I: Endocrinology, Bind 119, Nr. 5, 11.1986, s. 2376-82.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Schwartz, J, Billestrup, N, Perrin, M, Rivier, J & Vale, W 1986, 'Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF', Endocrinology, bind 119, nr. 5, s. 2376-82. https://doi.org/10.1210/endo-119-5-2376

APA

Schwartz, J., Billestrup, N., Perrin, M., Rivier, J., & Vale, W. (1986). Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF. Endocrinology, 119(5), 2376-82. https://doi.org/10.1210/endo-119-5-2376

Vancouver

Schwartz J, Billestrup N, Perrin M, Rivier J, Vale W. Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF. Endocrinology. 1986 nov.;119(5):2376-82. https://doi.org/10.1210/endo-119-5-2376

Author

Schwartz, J ; Billestrup, Nils ; Perrin, M ; Rivier, J ; Vale, W. / Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF. I: Endocrinology. 1986 ; Bind 119, Nr. 5. s. 2376-82.

Bibtex

@article{67f1a59953394f31b0f6fe7b2a577056,
title = "Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF",
abstract = "A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.",
keywords = "Adrenocorticotropic Hormone, Animals, Cattle, Corticotropin-Releasing Hormone, Dexamethasone, Dose-Response Relationship, Drug, Flow Cytometry, Fluorescent Dyes, Microscopy, Fluorescence, Pituitary Gland, Anterior, Rats",
author = "J Schwartz and Nils Billestrup and M Perrin and J Rivier and W Vale",
year = "1986",
month = nov,
doi = "10.1210/endo-119-5-2376",
language = "English",
volume = "119",
pages = "2376--82",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

AU - Schwartz, J

AU - Billestrup, Nils

AU - Perrin, M

AU - Rivier, J

AU - Vale, W

PY - 1986/11

Y1 - 1986/11

N2 - A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.

AB - A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.

KW - Adrenocorticotropic Hormone

KW - Animals

KW - Cattle

KW - Corticotropin-Releasing Hormone

KW - Dexamethasone

KW - Dose-Response Relationship, Drug

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Microscopy, Fluorescence

KW - Pituitary Gland, Anterior

KW - Rats

U2 - 10.1210/endo-119-5-2376

DO - 10.1210/endo-119-5-2376

M3 - Journal article

C2 - 3021441

VL - 119

SP - 2376

EP - 2382

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0013-7227

IS - 5

ER -

ID: 132900851