Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting
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Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting. / Jarolim, E; Matthiesen, F; Skov, P S; Tejkl, M; Oesterballe, O; Poulsen, L K.
I: International Archives of Allergy and Applied Immunology, Bind 92, Nr. 3, 1990, s. 254-64.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Hyposensitization therapy with whole pollen extract or purified allergens monitored by immunoblotting
AU - Jarolim, E
AU - Matthiesen, F
AU - Skov, P S
AU - Tejkl, M
AU - Oesterballe, O
AU - Poulsen, L K
PY - 1990
Y1 - 1990
N2 - Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.
AB - Patients allergic to grass pollen were hyposensitized with two major allergenic components or whole extract of timothy grass pollen. Specific IgE, IgG1, and IgG4 formed during immunotherapy were analyzed by immunoblotting. Similar antibody-binding patterns were observed in both patient groups. Inhibition experiments using allergenic components isolated by preparative sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that all antigenic components of timothy grass pollen detected in immunoblot dispose of private and cross-reactive determinants for binding of human IgE. The worse clinical outcome of immunotherapy after hyposensitization with two cross-reactive components did, therefore, not correlate with a specific antibody formation pattern.
KW - Allergens
KW - Antibody Affinity
KW - Cross Reactions
KW - Desensitization, Immunologic
KW - Electrophoresis, Polyacrylamide Gel
KW - Histamine Release
KW - Humans
KW - Immunoblotting
KW - Immunoglobulin E
KW - Immunoglobulin G
KW - Poaceae
KW - Pollen
KW - Journal Article
M3 - Journal article
C2 - 1703516
VL - 92
SP - 254
EP - 264
JO - International Archives of Allergy and Immunology
JF - International Archives of Allergy and Immunology
SN - 1018-2438
IS - 3
ER -
ID: 169715191