TY - JOUR

T1 - GPR183 is Dispensable for B1 cell Accumulation and Function, but Affects B2 cell Abundance, in the Omentum and Peritoneal Cavity

AU - Barington, Line

AU - Christensen, Liv von Voss

AU - Pedersen, Kristian Kåber

AU - Arfelt, Kristine Niss

AU - Roumain, Martin

AU - Jensen, Kristian Høj Reveles

AU - Kjær, Viktoria Madeline Skovgaard

AU - Daugvilaite, Viktorija

AU - Kearney, John F.

AU - Christensen, Jan Pravsgaard

AU - Hjortø, Gertrud Malene

AU - Muccioli, Giulio G.

AU - Holst, Peter Johannes

AU - Rosenkilde, Mette Marie

N1 - Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022

Y1 - 2022

N2 - B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abun-dant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a-or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.

AB - B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abun-dant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G protein-coupled receptor 183 (GPR183), also known as Epstein-Barr virus-induced gene 2 (EBI2), is an oxysterol-activated chemotactic receptor that regulates migration of B cells. We investigated the role of GPR183 in B1 cells in the peritoneal cavity and omentum. B1 cells expressed GPR183 at the mRNA level and migrated towards the GPR183 ligand 7α,25-dihydroxycholesterol (7α,25-OHC). GPR183 knock-out (KO) mice had smaller omenta, but with normal numbers of B1 cells, whereas they had fewer B2 cells in the omentum and peritoneal cavity than wildtype (WT) mice. GPR183 was not responsible for B1 cell accumulation in the omentum in response to i.p. lipopolysaccharide (LPS)-injection, in spite of a massive increase in 7α,25-OHC levels. Lack of GPR183 also did not affect B1a-or B1b cell-specific antibody responses after vaccination. In conclusion, we found that GPR183 is non-essential for the accumulation and function of B1 cells in the omentum and peritoneal cavity, but that it influences the abundance of B2 cells in these compartments.

KW - 7TM receptor

KW - B1 cell, B-1 cell

KW - GPCR

KW - GPR183, EBI2

KW - Oxysterol

UR - http://www.scopus.com/inward/record.url?scp=85123573326&partnerID=8YFLogxK

U2 - 10.3390/cells11030494

DO - 10.3390/cells11030494

M3 - Journal article

C2 - 35159303

AN - SCOPUS:85123573326

VL - 11

JO - Cells

JF - Cells

SN - 2073-4409

IS - 3

M1 - 494

ER -