Food-grade hexosomes as efficient vehicles for delivery of fish-purified antioxidant peptide
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Food-grade hexosomes as efficient vehicles for delivery of fish-purified antioxidant peptide. / Ramezanzade, Leila; Hosseini, Seyed Fakhreddin; Sajedi, Reza H.; Mirzai Nielsen, Amy; Yaghmur, Anan.
I: Food Chemistry, Bind 434, 137446, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Food-grade hexosomes as efficient vehicles for delivery of fish-purified antioxidant peptide
AU - Ramezanzade, Leila
AU - Hosseini, Seyed Fakhreddin
AU - Sajedi, Reza H.
AU - Mirzai Nielsen, Amy
AU - Yaghmur, Anan
N1 - Funding Information: The authors thank the Iran National Science Foundation (INSF; Grant No. 4014594), Biotechnology Development Council (BDC; Grant No. 01/17015), and the Vice-presidency for Research and Technology of Tarbiat Modares University (IG-39804) for the financial support. Publisher Copyright: © 2023 Elsevier Ltd
PY - 2024
Y1 - 2024
N2 - Herein, we describe the potential use of food-grade hexosomes (HEXs) for delivering fish-purified antioxidant peptide (PF10). Using a binary lipid mixture of Dimodan U/citrem, the nanocarriers were produced with a size range of 202.7–569.8 nm and peptide encapsulation efficiency of 64.6–89.3%. These HEXs were also characterized by SAXS and cryo-TEM, and were able to sustain the release of PF10, where only 32.2% released in PBS after 24 h. SAXS findings verified that PF10 modulate the internal structure of HEXs in a pH-dependent manner. Antioxidant assays proved the efficacy of such nano-self-assemblies in maintaining the bioactivity of the loaded peptide. Moreover, the in vitro gastrointestinal stability test indicated that the antioxidant capacity of the free- and PF10-loaded HEXs decreased under SGF/SIF conditions with the reduction in activity being greater for the free PF10. The present findings may provide a useful basis for development of pH-responsive nano-self-assemblies for delivery of antioxidant peptides.
AB - Herein, we describe the potential use of food-grade hexosomes (HEXs) for delivering fish-purified antioxidant peptide (PF10). Using a binary lipid mixture of Dimodan U/citrem, the nanocarriers were produced with a size range of 202.7–569.8 nm and peptide encapsulation efficiency of 64.6–89.3%. These HEXs were also characterized by SAXS and cryo-TEM, and were able to sustain the release of PF10, where only 32.2% released in PBS after 24 h. SAXS findings verified that PF10 modulate the internal structure of HEXs in a pH-dependent manner. Antioxidant assays proved the efficacy of such nano-self-assemblies in maintaining the bioactivity of the loaded peptide. Moreover, the in vitro gastrointestinal stability test indicated that the antioxidant capacity of the free- and PF10-loaded HEXs decreased under SGF/SIF conditions with the reduction in activity being greater for the free PF10. The present findings may provide a useful basis for development of pH-responsive nano-self-assemblies for delivery of antioxidant peptides.
KW - Antioxidant peptide delivery
KW - Citrem
KW - Cryo-TEM
KW - Hexosomes
KW - In vitro gastrointestinal digestion
KW - SAXS
U2 - 10.1016/j.foodchem.2023.137446
DO - 10.1016/j.foodchem.2023.137446
M3 - Journal article
C2 - 37722336
AN - SCOPUS:85171474128
VL - 434
JO - Food Chemistry
JF - Food Chemistry
SN - 0308-8146
M1 - 137446
ER -
ID: 375672946