Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres

Publikation: Working paper › Preprint › Forskning

Standard

Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres. / Hoegsbjerg, Christian; Moebjerg, Ask; Yeung, Ching-Yan Chloe; Schjerling, Peter; Krogsgaard, Michael R; Koch, Manuel; Kjaer, Michael; Keudell, Arvind G von; Mackey, Abigail L.

bioRxiv, 2024.

Publikation: Working paper › Preprint › Forskning

Harvard

Hoegsbjerg, C, Moebjerg, A, Yeung, C-YC, Schjerling, P, Krogsgaard, MR, Koch, M, Kjaer, M, Keudell, AGV & Mackey, AL 2024 'Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres' bioRxiv. https://doi.org/10.1101/2024.05.03.592365

APA

Hoegsbjerg, C., Moebjerg, A., Yeung, C-Y. C., Schjerling, P., Krogsgaard, M. R., Koch, M., Kjaer, M., Keudell, A. G. V., & Mackey, A. L. (2024). Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres. bioRxiv. bioRxiv https://doi.org/10.1101/2024.05.03.592365

Vancouver

Hoegsbjerg C, Moebjerg A, Yeung C-YC, Schjerling P, Krogsgaard MR, Koch M o.a. Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres. bioRxiv. 2024 jan. 1. https://doi.org/10.1101/2024.05.03.592365

Author

Hoegsbjerg, Christian ; Moebjerg, Ask ; Yeung, Ching-Yan Chloe ; Schjerling, Peter ; Krogsgaard, Michael R ; Koch, Manuel ; Kjaer, Michael ; Keudell, Arvind G von ; Mackey, Abigail L. / Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres. bioRxiv, 2024. (bioRxiv).

Bibtex

@techreport{aae2879dada24f5790dcf5db42171a0e,
title = "Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres",
abstract = "The myotendinous junction (MTJ) is a weak link in the musculoskeletal system. Here, we isolated the tips of single myofibres from healthy human hamstring muscles for confocal microscopy (n=6) and RNAscope in situ hybridization (n=6) to gain insight into the profiles of cells and myonuclei in this region. A marked presence of mononuclear cells was observed coating the fibre tips, with a median of 29 (range 16-63) and 16 (9-23) cells per fibre for type I and II myofibres, respectively (p<0.05). The number and density of myonuclei gradually increased from the myofibre proper towards the tip (p<0.05), similarly for both fibre types, and a greater number of COL22A1-expressing nuclei was seen in type II vs type I myofibres (p<0.05). These divergent fibre type-specific characteristics of the MTJ reflect the respective demands for remodelling of the tendon and myofibre sides of the junction according to loading patterns. This insight refines our fundamental understanding of the human MTJ at the cell and structural levels.Competing Interest StatementThe authors have declared no competing interest.",
author = "Christian Hoegsbjerg and Ask Moebjerg and Yeung, {Ching-Yan Chloe} and Peter Schjerling and Krogsgaard, {Michael R} and Manuel Koch and Michael Kjaer and Keudell, {Arvind G von} and Mackey, {Abigail L}",
year = "2024",
month = jan,
day = "1",
doi = "10.1101/2024.05.03.592365",
language = "English",
series = "bioRxiv",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres

AU - Hoegsbjerg, Christian

AU - Moebjerg, Ask

AU - Yeung, Ching-Yan Chloe

AU - Schjerling, Peter

AU - Krogsgaard, Michael R

AU - Koch, Manuel

AU - Kjaer, Michael

AU - Keudell, Arvind G von

AU - Mackey, Abigail L

PY - 2024/1/1

Y1 - 2024/1/1

N2 - The myotendinous junction (MTJ) is a weak link in the musculoskeletal system. Here, we isolated the tips of single myofibres from healthy human hamstring muscles for confocal microscopy (n=6) and RNAscope in situ hybridization (n=6) to gain insight into the profiles of cells and myonuclei in this region. A marked presence of mononuclear cells was observed coating the fibre tips, with a median of 29 (range 16-63) and 16 (9-23) cells per fibre for type I and II myofibres, respectively (p<0.05). The number and density of myonuclei gradually increased from the myofibre proper towards the tip (p<0.05), similarly for both fibre types, and a greater number of COL22A1-expressing nuclei was seen in type II vs type I myofibres (p<0.05). These divergent fibre type-specific characteristics of the MTJ reflect the respective demands for remodelling of the tendon and myofibre sides of the junction according to loading patterns. This insight refines our fundamental understanding of the human MTJ at the cell and structural levels.Competing Interest StatementThe authors have declared no competing interest.

AB - The myotendinous junction (MTJ) is a weak link in the musculoskeletal system. Here, we isolated the tips of single myofibres from healthy human hamstring muscles for confocal microscopy (n=6) and RNAscope in situ hybridization (n=6) to gain insight into the profiles of cells and myonuclei in this region. A marked presence of mononuclear cells was observed coating the fibre tips, with a median of 29 (range 16-63) and 16 (9-23) cells per fibre for type I and II myofibres, respectively (p<0.05). The number and density of myonuclei gradually increased from the myofibre proper towards the tip (p<0.05), similarly for both fibre types, and a greater number of COL22A1-expressing nuclei was seen in type II vs type I myofibres (p<0.05). These divergent fibre type-specific characteristics of the MTJ reflect the respective demands for remodelling of the tendon and myofibre sides of the junction according to loading patterns. This insight refines our fundamental understanding of the human MTJ at the cell and structural levels.Competing Interest StatementThe authors have declared no competing interest.

U2 - 10.1101/2024.05.03.592365

DO - 10.1101/2024.05.03.592365

M3 - Preprint

T3 - bioRxiv

BT - Fibre type differences in the organisation of mononuclear cells and myonuclei at the tips of human myofibres

PB - bioRxiv

ER -

ID: 392878348