Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque
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Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque. / Fu, S; Davies, Michael Jonathan; Stocker, R; Dean, R T.
I: Biochemical Journal, Bind 333 ( Pt 3), 01.08.1998, s. 519-25.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Evidence for roles of radicals in protein oxidation in advanced human atherosclerotic plaque
AU - Fu, S
AU - Davies, Michael Jonathan
AU - Stocker, R
AU - Dean, R T
PY - 1998/8/1
Y1 - 1998/8/1
N2 - Oxidative damage might be important in atherogenesis. Oxidized lipids are present at significant concentrations in advanced human plaque, although tissue antioxidants are mostly present at normal concentrations. Indirect evidence of protein modification (notably derivatization of lysine) or oxidation has been obtained by immunochemical methods; the specificities of these antibodies are unclear. Here we present chemical determinations of six protein-bound oxidation products: dopa, o-tyrosine, m-tyrosine, dityrosine, hydroxyleucine and hydroxyvaline, some of which reflect particularly oxy-radical-mediated reaction pathways, which seem to involve mainly the participation of transition- metal ions. We compared the relative abundance of these oxidation products in normal intima, and in human carotid plaque samples with that observed after radiolytically generated hydroxyl radical attack on BSA in vitro. The close similarities in relative abundances in the latter two circumstances indicate that hydroxyl radical damage might occur in plaque. The relatively higher level of dityrosine in plaque than that observed after radiolysis suggests the additional involvement of HOCl-mediated reactions in advanced plaque.
AB - Oxidative damage might be important in atherogenesis. Oxidized lipids are present at significant concentrations in advanced human plaque, although tissue antioxidants are mostly present at normal concentrations. Indirect evidence of protein modification (notably derivatization of lysine) or oxidation has been obtained by immunochemical methods; the specificities of these antibodies are unclear. Here we present chemical determinations of six protein-bound oxidation products: dopa, o-tyrosine, m-tyrosine, dityrosine, hydroxyleucine and hydroxyvaline, some of which reflect particularly oxy-radical-mediated reaction pathways, which seem to involve mainly the participation of transition- metal ions. We compared the relative abundance of these oxidation products in normal intima, and in human carotid plaque samples with that observed after radiolytically generated hydroxyl radical attack on BSA in vitro. The close similarities in relative abundances in the latter two circumstances indicate that hydroxyl radical damage might occur in plaque. The relatively higher level of dityrosine in plaque than that observed after radiolysis suggests the additional involvement of HOCl-mediated reactions in advanced plaque.
KW - Adult
KW - Amino Acids
KW - Arteriosclerosis
KW - Blood Proteins
KW - Dihydroxyphenylalanine
KW - Female
KW - Humans
KW - Hydroxides
KW - Hydroxyl Radical
KW - Hypochlorous Acid
KW - Male
KW - Middle Aged
KW - Oxidation-Reduction
KW - Peroxides
KW - Proteins
KW - Serum Albumin, Bovine
KW - Tunica Intima
M3 - Journal article
C2 - 9677308
VL - 333 ( Pt 3)
SP - 519
EP - 525
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
ER -
ID: 138283775